Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMV_RS08070 Genome accession   NC_017501
Coordinates   1576250..1577380 (-) Length   376 a.a.
NCBI ID   WP_002232075.1    Uniprot ID   C6S635
Organism   Neisseria meningitidis 8013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571250..1582380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS08025 (NMV_1619) - 1571700..1572122 (-) 423 WP_002213999.1 protein Gly1ORF1 -
  NMV_RS08030 (NMV_1620) - 1572373..1572693 (-) 321 WP_002214001.1 DUF2322 family protein -
  NMV_RS08035 (NMV_1621) upp 1572716..1573342 (-) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  NMV_RS08045 (NMV_1622) grxD 1573448..1573759 (-) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMV_RS08050 - 1573860..1574075 (-) 216 WP_002219531.1 putative hemolysin -
  NMV_RS08055 (NMV_1623) - 1574101..1574880 (-) 780 WP_002224604.1 TatD family hydrolase -
  NMV_RS08060 (NMV_1624) - 1574885..1575235 (-) 351 WP_002214009.1 PilZ domain-containing protein -
  NMV_RS08065 (NMV_1625) - 1575239..1576216 (-) 978 WP_002222732.1 DNA polymerase III subunit delta' -
  NMV_RS08070 (NMV_1626) pilU 1576250..1577380 (-) 1131 WP_002232075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMV_RS08075 (NMV_1627) - 1577548..1578249 (+) 702 WP_002240423.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMV_RS08080 (NMV_1628) lepA 1578392..1580185 (+) 1794 WP_002237819.1 translation elongation factor 4 -
  NMV_RS08085 (NMV_1629) lepB 1580328..1581347 (+) 1020 WP_002243408.1 signal peptidase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2885

>NTDB_id=47750 NMV_RS08070 WP_002232075.1 1576250..1577380(-) (pilU) [Neisseria meningitidis 8013]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=47750 NMV_RS08070 WP_002232075.1 1576250..1577380(-) (pilU) [Neisseria meningitidis 8013]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACTCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6S635

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment