Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   H7793_RS02950 Genome accession   NZ_CP060642
Coordinates   563903..565228 (+) Length   441 a.a.
NCBI ID   WP_002988971.1    Uniprot ID   Q1JKR0
Organism   Streptococcus pyogenes strain TSPY1312     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 563903..587459 563903..565228 within 0


Gene organization within MGE regions


Location: 563903..587459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7793_RS02950 (H7793_02965) comFA/cflA 563903..565228 (+) 1326 WP_002988971.1 DEAD/DEAH box helicase Machinery gene
  H7793_RS02955 (H7793_02970) - 565200..565865 (+) 666 WP_002983685.1 ComF family protein -
  H7793_RS02960 (H7793_02975) hpf 565945..566493 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  H7793_RS02965 (H7793_02980) - 567209..567523 (+) 315 WP_000420682.1 YdcP family protein -
  H7793_RS02970 (H7793_02985) - 567539..567925 (+) 387 WP_000985015.1 YdcP family protein -
  H7793_RS02975 (H7793_02990) - 567954..569339 (+) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  H7793_RS02980 (H7793_02995) - 569342..569482 (+) 141 WP_074186995.1 conjugal transfer protein -
  H7793_RS02985 (H7793_03000) mobT 569517..570719 (+) 1203 Protein_540 MobT family relaxase -
  H7793_RS09100 - 570768..570935 (+) 168 WP_000502600.1 MLS leader peptide -
  H7793_RS02990 (H7793_03005) - 570984..571067 (+) 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
  H7793_RS02995 (H7793_03010) erm(B) 571192..571929 (+) 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
  H7793_RS03000 (H7793_03015) - 572186..573433 (-) 1248 WP_000019067.1 ISL3 family transposase -
  H7793_RS03005 (H7793_03020) - 573613..573834 (+) 222 WP_001009056.1 hypothetical protein -
  H7793_RS03010 (H7793_03025) - 573951..574448 (+) 498 WP_000342539.1 antirestriction protein ArdA -
  H7793_RS03015 (H7793_03030) - 574537..574929 (+) 393 WP_000723888.1 conjugal transfer protein -
  H7793_RS03020 (H7793_03035) - 574913..577360 (+) 2448 WP_228629717.1 ATP-binding protein -
  H7793_RS03025 (H7793_03040) - 577363..579540 (+) 2178 WP_000804748.1 CD3337/EF1877 family mobilome membrane protein -
  H7793_RS03030 (H7793_03045) - 579537..580418 (+) 882 WP_006150747.1 lysozyme family protein -
  H7793_RS03035 (H7793_03050) - 580415..581347 (+) 933 WP_001224318.1 conjugal transfer protein -
  H7793_RS09205 - 581661..581708 (+) 48 Protein_552 hypothetical protein -
  H7793_RS03045 (H7793_03060) tet(M) 581724..583643 (+) 1920 WP_000691736.1 tetracycline resistance ribosomal protection protein Tet(M) -
  H7793_RS03050 (H7793_03065) - 583762..583929 (+) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  H7793_RS03055 (H7793_03070) - 583989..584342 (-) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  H7793_RS03060 (H7793_03075) - 584847..585269 (+) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  H7793_RS03065 (H7793_03080) - 585266..585496 (+) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  H7793_RS03070 - 585722..585973 (-) 252 WP_001845478.1 hypothetical protein -
  H7793_RS03075 (H7793_03085) - 585957..586160 (+) 204 WP_000814511.1 excisionase -
  H7793_RS03080 (H7793_03090) - 586242..587459 (+) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49846.34 Da        Isoelectric Point: 10.0630

>NTDB_id=477145 H7793_RS02950 WP_002988971.1 563903..565228(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY1312]
MESIENSYGRLLLENQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSNKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=477145 H7793_RS02950 WP_002988971.1 563903..565228(+) (comFA/cflA) [Streptococcus pyogenes strain TSPY1312]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAACCAGTTACCAGATTCAGCAAGGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAAATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1JKR0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae D39

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae R6

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae TIGR4

54.415

95.011

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

53.081

95.692

0.508
  comFA/cflA Streptococcus mitis SK321

52.133

95.692

0.499
  comFA Lactococcus lactis subsp. cremoris KW2

47.118

90.476

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365