Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J0G23_RS10450 Genome accession   NZ_CP071263
Coordinates   2118913..2119722 (-) Length   269 a.a.
NCBI ID   WP_024230005.1    Uniprot ID   -
Organism   Escherichia coli strain MEI003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2113913..2124722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G23_RS10435 (J0G23_10365) gspD 2115370..2117430 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  J0G23_RS10440 (J0G23_10370) gspC 2117460..2118419 (-) 960 WP_024230003.1 type II secretion system protein GspC -
  J0G23_RS10445 (J0G23_10375) gspS2 2118437..2118847 (-) 411 WP_024230004.1 type II secretion system pilot lipoprotein GspS-beta -
  J0G23_RS10450 (J0G23_10380) pilD 2118913..2119722 (-) 810 WP_024230005.1 prepilin peptidase PppA Machinery gene
  J0G23_RS10455 (J0G23_10385) sslE 2119862..2124424 (-) 4563 WP_024230006.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29416.01 Da        Isoelectric Point: 8.0831

>NTDB_id=476435 J0G23_RS10450 WP_024230005.1 2118913..2119722(-) (pilD) [Escherichia coli strain MEI003]
MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSTQSKISLALPCSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=476435 J0G23_RS10450 WP_024230005.1 2118913..2119722(-) (pilD) [Escherichia coli strain MEI003]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCGTCCTGGCTACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTACGC
AGTCAAAAATAAGCCTTGCGTTGCCGTGTTCGCACTGCCCACATTGTCAGCAGACCATCCGCGTTCGCGACAACATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTGTGGCCGGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGTGTCATCGACCTCGATAACCAATGGCTGCCCGATGTCTTTACTCAGGGCGTATTGTGGACG
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACACTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCTCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGGCGTTATCACTGCCCAACGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Neisseria gonorrhoeae MS11

39.689

95.539

0.379
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368