Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilB   Type   Machinery gene
Locus tag   H8Z71_RS05240 Genome accession   NZ_CP060465
Coordinates   1227170..1227289 (+) Length   39 a.a.
NCBI ID   WP_005915819.1    Uniprot ID   A0A7S6YV99
Organism   Xanthomonas citri pv. citri strain DAR73886     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1227518..1249155 1227170..1227289 flank 229


Gene organization within MGE regions


Location: 1227170..1249155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8Z71_RS05240 (H8Z71_05240) pilB 1227170..1227289 (+) 120 WP_005915819.1 hypothetical protein Machinery gene
  H8Z71_RS05245 (H8Z71_05245) - 1227421..1227588 (+) 168 WP_015463523.1 hypothetical protein -
  H8Z71_RS05250 (H8Z71_05250) pilR 1227811..1229205 (-) 1395 WP_005930970.1 sigma-54 dependent transcriptional regulator Regulator
  H8Z71_RS05255 (H8Z71_05255) - 1229530..1231143 (-) 1614 WP_003488599.1 HAMP domain-containing sensor histidine kinase -
  H8Z71_RS05260 (H8Z71_05260) sucC 1231375..1232544 (+) 1170 WP_005915812.1 ADP-forming succinate--CoA ligase subunit beta -
  H8Z71_RS05265 (H8Z71_05265) sucD 1232569..1233444 (+) 876 WP_005921370.1 succinate--CoA ligase subunit alpha -
  H8Z71_RS23320 - 1233531..1234046 (+) 516 WP_011052123.1 hypothetical protein -
  H8Z71_RS05275 (H8Z71_05275) - 1234001..1235116 (-) 1116 WP_011052122.1 IS1595 family transposase -
  H8Z71_RS05280 (H8Z71_05280) - 1235344..1235628 (-) 285 WP_016849322.1 hypothetical protein -
  H8Z71_RS05285 (H8Z71_05285) - 1235733..1236539 (+) 807 WP_015463520.1 hypothetical protein -
  H8Z71_RS05290 (H8Z71_05290) xopAI 1236959..1237849 (-) 891 WP_076605129.1 type III secretion system effector XopAI -
  H8Z71_RS05295 (H8Z71_05295) - 1237983..1239085 (-) 1103 Protein_1041 DNA-binding protein -
  H8Z71_RS05300 (H8Z71_05300) xopE 1239416..1240492 (-) 1077 WP_041471283.1 XopE/AvrPphe family type III secretion system effector -
  H8Z71_RS05305 (H8Z71_05305) - 1240626..1241709 (-) 1084 Protein_1043 DNA-binding protein -
  H8Z71_RS05310 (H8Z71_05310) - 1241902..1243175 (+) 1274 Protein_1044 site-specific integrase -
  H8Z71_RS05315 (H8Z71_05315) - 1243184..1246174 (+) 2991 WP_015472512.1 Tn3 family transposase -
  H8Z71_RS05320 (H8Z71_05320) - 1246307..1247584 (+) 1278 WP_005919631.1 lytic murein transglycosylase -
  H8Z71_RS05325 (H8Z71_05325) avrXacE2 1247669..1248739 (+) 1071 WP_011052114.1 type III secretion system effector avirulence protein AvrXacE2 -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4178.84 Da        Isoelectric Point: 9.0113

>NTDB_id=476264 H8Z71_RS05240 WP_005915819.1 1227170..1227289(+) (pilB) [Xanthomonas citri pv. citri strain DAR73886]
MQIAEAAQAIGIRDLRQSALMKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 120 bp        

>NTDB_id=476264 H8Z71_RS05240 WP_005915819.1 1227170..1227289(+) (pilB) [Xanthomonas citri pv. citri strain DAR73886]
ATGCAGATCGCCGAGGCCGCACAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATGAAGGCTGCGCACGGGGT
GACCAGCCTGGCGGAGATCAATCGTGTGACGAAGGACTAA

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A7S6YV99

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.282

100

0.513

  pilB Acinetobacter baumannii D1279779

48.718

100

0.487

  pilB Vibrio cholerae strain A1552

45.714

89.744

0.41

  pilB Legionella pneumophila strain ERS1305867

38.462

100

0.385