Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   H8S71_RS18550 Genome accession   NZ_CP060417
Coordinates   3631311..3632456 (+) Length   381 a.a.
NCBI ID   WP_125121077.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3626311..3637456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS18530 (H8S71_18525) albF 3626327..3627607 (+) 1281 WP_041850411.1 pitrilysin family protein -
  H8S71_RS18535 (H8S71_18530) albG 3627604..3628305 (+) 702 WP_187001947.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  H8S71_RS18540 (H8S71_18535) ywhL 3628311..3629687 (-) 1377 WP_125121079.1 YncE family protein -
  H8S71_RS18545 (H8S71_18540) ywhK 3629726..3631081 (-) 1356 WP_038829813.1 YncE family protein -
  H8S71_RS20660 - 3631078..3631308 (-) 231 WP_125121078.1 hypothetical protein -
  H8S71_RS18550 (H8S71_18545) rapF 3631311..3632456 (+) 1146 WP_125121077.1 response regulator aspartate phosphatase RapF Regulator
  H8S71_RS18555 (H8S71_18550) phrF 3632440..3632559 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  H8S71_RS18560 (H8S71_18555) - 3632814..3633293 (+) 480 WP_103804110.1 hypothetical protein -
  H8S71_RS18565 (H8S71_18560) - 3633444..3634232 (+) 789 WP_038829810.1 membrane protein -
  H8S71_RS18570 (H8S71_18565) - 3634255..3634893 (+) 639 WP_205831735.1 hypothetical protein -
  H8S71_RS18575 (H8S71_18570) - 3634909..3635700 (+) 792 WP_250623939.1 hypothetical protein -
  H8S71_RS18580 (H8S71_18575) - 3635703..3636350 (+) 648 WP_015714896.1 ABC transporter ATP-binding protein -
  H8S71_RS18585 (H8S71_18580) - 3636343..3636903 (+) 561 WP_015714897.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45676.15 Da        Isoelectric Point: 4.9912

>NTDB_id=475925 H8S71_RS18550 WP_125121077.1 3631311..3632456(+) (rapF) [Bacillus subtilis subsp. subtilis strain ONU 559]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAEDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=475925 H8S71_RS18550 WP_125121077.1 3631311..3632456(+) (rapF) [Bacillus subtilis subsp. subtilis strain ONU 559]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAGTATTTGCGAAGAGAAATCAAACAAGAGCTGGATCAAATGGAAGAAGATCAGGATCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGAAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(223-290)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

99.213

100

0.992
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572