Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H8S71_RS12130 Genome accession   NZ_CP060417
Coordinates   2382520..2383590 (-) Length   356 a.a.
NCBI ID   WP_015714258.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2377520..2388590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS12085 (H8S71_12080) tapA 2377998..2378759 (-) 762 WP_128993152.1 amyloid fiber anchoring/assembly protein TapA -
  H8S71_RS12090 (H8S71_12085) yqzG 2379031..2379357 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  H8S71_RS12095 (H8S71_12090) spoIITA 2379399..2379578 (-) 180 WP_014480252.1 YqzE family protein -
  H8S71_RS12100 (H8S71_12095) comGG 2379650..2380024 (-) 375 WP_128993153.1 ComG operon protein ComGG Machinery gene
  H8S71_RS12105 (H8S71_12100) comGF 2380025..2380408 (-) 384 WP_029317913.1 ComG operon protein ComGF Machinery gene
  H8S71_RS12110 (H8S71_12105) comGE 2380434..2380781 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  H8S71_RS12115 (H8S71_12110) comGD 2380765..2381196 (-) 432 WP_015714255.1 comG operon protein ComGD Machinery gene
  H8S71_RS12120 (H8S71_12115) comGC 2381186..2381482 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  H8S71_RS12125 (H8S71_12120) comGB 2381496..2382533 (-) 1038 WP_072175548.1 comG operon protein ComGB Machinery gene
  H8S71_RS12130 (H8S71_12125) comGA 2382520..2383590 (-) 1071 WP_015714258.1 competence protein ComGA Machinery gene
  H8S71_RS12135 (H8S71_12130) corA 2384002..2384955 (-) 954 WP_015483432.1 magnesium transporter CorA -
  H8S71_RS12140 (H8S71_12135) yqhB 2385098..2386426 (+) 1329 WP_014480261.1 hemolysin family protein -
  H8S71_RS12145 (H8S71_12140) rsbRD 2386479..2387315 (-) 837 WP_021480027.1 RsbT co-antagonist protein RsbRD -
  H8S71_RS12155 (H8S71_12150) mgsR 2387539..2387919 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  H8S71_RS12160 (H8S71_12155) yqgY 2388152..2388397 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40488.67 Da        Isoelectric Point: 9.0175

>NTDB_id=475899 H8S71_RS12130 WP_015714258.1 2382520..2383590(-) (comGA) [Bacillus subtilis subsp. subtilis strain ONU 559]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=475899 H8S71_RS12130 WP_015714258.1 2382520..2383590(-) (comGA) [Bacillus subtilis subsp. subtilis strain ONU 559]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCACAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCACTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997