Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   H8S71_RS08620 Genome accession   NZ_CP060417
Coordinates   1653544..1654437 (+) Length   297 a.a.
NCBI ID   WP_015715882.1    Uniprot ID   A0A8E0SFH2
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1648544..1659437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS08600 (H8S71_08595) - 1649214..1650944 (+) 1731 WP_187002227.1 hypothetical protein -
  H8S71_RS08605 (H8S71_08600) ylqH 1650941..1651222 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  H8S71_RS08610 (H8S71_08605) sucC 1651395..1652552 (+) 1158 WP_169037510.1 ADP-forming succinate--CoA ligase subunit beta -
  H8S71_RS08615 (H8S71_08610) sucD 1652581..1653483 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  H8S71_RS08620 (H8S71_08615) dprA 1653544..1654437 (+) 894 WP_015715882.1 DNA-processing protein DprA Machinery gene
  H8S71_RS08625 (H8S71_08620) topA 1654625..1656700 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  H8S71_RS08630 (H8S71_08625) trmFO 1656776..1658083 (+) 1308 WP_015715883.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  H8S71_RS08635 (H8S71_08630) xerC 1658151..1659065 (+) 915 WP_003231988.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32904.21 Da        Isoelectric Point: 9.9142

>NTDB_id=475884 H8S71_RS08620 WP_015715882.1 1653544..1654437(+) (dprA) [Bacillus subtilis subsp. subtilis strain ONU 559]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=475884 H8S71_RS08620 WP_015715882.1 1653544..1654437(+) (dprA) [Bacillus subtilis subsp. subtilis strain ONU 559]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAGCACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

98.99

100

0.99
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377