Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H7696_RS01565 Genome accession   NZ_CP060386
Coordinates   333735..334328 (+) Length   197 a.a.
NCBI ID   WP_033907305.1    Uniprot ID   A0AAW9B9Y8
Organism   Vibrio alginolyticus strain Vb1833     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328735..339328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7696_RS01550 (H7696_01550) - 329461..332022 (-) 2562 WP_085570127.1 penicillin-binding protein 1A -
  H7696_RS01555 (H7696_01555) pilM 332167..333183 (+) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  H7696_RS01560 (H7696_01560) pilN 333167..333742 (+) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  H7696_RS01565 (H7696_01565) pilO 333735..334328 (+) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  H7696_RS01570 (H7696_01570) pilP 334318..334833 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  H7696_RS01575 (H7696_01575) pilQ 334859..336604 (+) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  H7696_RS01580 (H7696_01580) aroK 336792..337310 (+) 519 WP_005381319.1 shikimate kinase AroK -
  H7696_RS01585 (H7696_01585) aroB 337343..338443 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23001.51 Da        Isoelectric Point: 5.0948

>NTDB_id=475558 H7696_RS01565 WP_033907305.1 333735..334328(+) (pilO) [Vibrio alginolyticus strain Vb1833]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=475558 H7696_RS01565 WP_033907305.1 333735..334328(+) (pilO) [Vibrio alginolyticus strain Vb1833]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGTGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685