Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JR909_RS18835 Genome accession   NZ_CP070920
Coordinates   3878967..3879407 (+) Length   146 a.a.
NCBI ID   WP_000360927.1    Uniprot ID   -
Organism   Escherichia coli strain 40EC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3873967..3884407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR909_RS18815 (JR909_18815) aroP 3874965..3876338 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  JR909_RS18820 (JR909_18820) ampE 3876381..3877235 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  JR909_RS18825 (JR909_18825) ampD 3877232..3877783 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JR909_RS18830 (JR909_18830) nadC 3877871..3878764 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JR909_RS18835 (JR909_18835) pilA 3878967..3879407 (+) 441 WP_000360927.1 prepilin peptidase-dependent pilin Machinery gene
  JR909_RS18840 (JR909_18840) pilB 3879417..3880802 (+) 1386 WP_001025170.1 type II secretion system protein GspE Machinery gene
  JR909_RS18845 (JR909_18845) hofC 3880792..3881994 (+) 1203 WP_000157236.1 protein transport protein HofC -
  JR909_RS18850 (JR909_18850) guaC 3882029..3883072 (-) 1044 WP_001217338.1 GMP reductase -
  JR909_RS18855 (JR909_18855) - 3883228..3883272 (-) 45 WP_120795372.1 protein YacM -
  JR909_RS18860 (JR909_18860) coaE 3883297..3883917 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15533.60 Da        Isoelectric Point: 4.2529

>NTDB_id=475067 JR909_RS18835 WP_000360927.1 3878967..3879407(+) (pilA) [Escherichia coli strain 40EC]
MDKQSGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=475067 JR909_RS18835 WP_000360927.1 3878967..3879407(+) (pilA) [Escherichia coli strain 40EC]
ATGGACAAGCAAAGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

42.742

84.932

0.363