Detailed information NTG0047473

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: I653_RS08800
• Genome accession: NC_020832
• Coordinates: 1735009..1737585 (+)
• Length: 858 a.a.
• NCBI ID: WP_015483239.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis str. BAB-1
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1730009..1742585

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I653_RS08780 (I653_08720)	kbl	1730781..1731959 (+)	1179	WP_015383694.1	glycine C-acetyltransferase	-
I653_RS08785 (I653_08725)	miaB	1732107..1733636 (+)	1530	WP_015483238.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
I653_RS08790 (I653_08730)	ymcA	1733638..1734069 (+)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
I653_RS08795 (I653_08735)	cotE	1734331..1734876 (+)	546	WP_003231833.1	outer spore coat protein CotE	-
I653_RS08800 (I653_08740)	hexA	1735009..1737585 (+)	2577	WP_015483239.1	DNA mismatch repair protein MutS	Machinery gene
I653_RS08805 (I653_08745)	hexB	1737601..1739484 (+)	1884	WP_015483240.1	DNA mismatch repair endonuclease MutL	Machinery gene
I653_RS08810 (I653_08750)	ymzD	1739884..1740339 (-)	456	WP_015483241.1	regulatory YrvL family protein	-
I653_RS08815 (I653_08755)	-	1740494..1741051 (-)	558	WP_015483242.1	hypothetical protein	-
I653_RS08820 (I653_08760)	pksA	1741172..1741789 (+)	618	WP_015383700.1	TetR/AcrR family transcriptional regulator	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97654.73 Da
• Isoelectric Point: 5.1585

>NTDB_id=47473 I653_RS08800 WP_015483239.1 1735009..1737585(+) (hexA) [Bacillus subtilis subsp. subtilis str. BAB-1]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFMRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAERIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQQLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPMQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=47473 I653_RS08800 WP_015483239.1 1735009..1737585(+) (hexA) [Bacillus subtilis subsp. subtilis str. BAB-1]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTATGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGATG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGGTACAAAGTGGCAATCTGTGAACAAACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTGGTTCAGCT
GATTACGCCTGGAACTGTTATGGACGGCAAAGGCATCCATGAATCGGAAAATAACTTTATCGCATCTGTTTCAGCTTGCT
CGAACGGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACGATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTATGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTATACTCAAAGCGTAATCTGGAGCTGACCGAAACGATCCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATAAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTGATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTATATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTTGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTTCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAGAGCGCATTGACCCTTGCGGGGACGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGTCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGTTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAATTTGCACTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCAGAGCGCTACATTACACCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGTGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTACAGCAGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGTCACTACACGAAACCGGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCAAAAAAAGCGGTGCTTCCGATTTTCGATCAA
ATTTTTACGCGAATCGGTGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAACGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATTAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCACGCGCTCAAGATATTTTAAAAGAGCTTGAGCATTCAGGAAACAAACCGGAAGTGCCGATGCAGAAGCCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain III (MutS_III) (258-547)

• MutS domain II (MutS_II) (125-239)

• MutS domain I (MutS_I) (5-116)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.591	99.65	0.494
mutS	Pseudomonas stutzeri strain ATCC 17587	39.135	100	0.401