Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H7F27_RS20350 Genome accession   NZ_CP060193
Coordinates   4034959..4036335 (-) Length   458 a.a.
NCBI ID   WP_044153447.1    Uniprot ID   A0A9Q2LD59
Organism   Bacillus sp. PAMC26543     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4029959..4041335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F27_RS20330 (H7F27_20330) ispF 4031471..4031947 (-) 477 WP_103672508.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  H7F27_RS20335 (H7F27_20335) ispD 4031940..4032638 (-) 699 WP_185848355.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  H7F27_RS20340 (H7F27_20340) - 4032653..4033753 (-) 1101 WP_059291688.1 PIN/TRAM domain-containing protein -
  H7F27_RS20345 (H7F27_20345) disA 4033873..4034955 (-) 1083 WP_003242042.1 DNA integrity scanning diadenylate cyclase DisA -
  H7F27_RS20350 (H7F27_20350) radA 4034959..4036335 (-) 1377 WP_044153447.1 DNA repair protein RadA Machinery gene
  H7F27_RS20355 (H7F27_20355) clpC 4036427..4038859 (-) 2433 WP_010332750.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  H7F27_RS20360 (H7F27_20360) - 4038856..4039947 (-) 1092 WP_059291687.1 protein arginine kinase -
  H7F27_RS20365 (H7F27_20365) - 4039947..4040504 (-) 558 WP_095714843.1 UvrB/UvrC motif-containing protein -
  H7F27_RS20370 (H7F27_20370) ctsR 4040518..4040982 (-) 465 WP_010332747.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49532.07 Da        Isoelectric Point: 8.2443

>NTDB_id=474543 H7F27_RS20350 WP_044153447.1 4034959..4036335(-) (radA) [Bacillus sp. PAMC26543]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSTRHVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIEEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=474543 H7F27_RS20350 WP_044153447.1 4034959..4036335(-) (radA) [Bacillus sp. PAMC26543]
ATGGCTAAAACAAAATCCAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACCGTACAGAAACCTTCCCCCATAACATCAATCGAAACATCAGAAGAACCCCGGGTTAAAACACAGCTCGGCGAGTTT
AACAGAGTGCTCGGCGGCGGTGTGGTGAAGGGCTCACTTGTTTTAATTGGCGGGGACCCCGGCATCGGAAAATCGACCTT
ATTACTGCAAGTATCCGCTCAGTTATCTGGTTCAACACGTCATGTCCTGTACATTTCAGGAGAAGAATCGGTAAAACAAA
CAAAATTAAGAGCAGACCGTCTGGGCATAAATAATCCCTCACTGCATGTTTTATCCGAAACCGATATGGAGTATATTTCG
TCTGCTATAGAAGAGATGAATCCAGCGTTTGTGGTTGTTGACTCTATTCAAACGGTCTATCAAAGCGATATTACATCGGC
TCCCGGCAGTGTGTCACAGGTAAGAGAATGTACAGCCGAGCTGATGAAAATTGCAAAGACAAAAGGTATTCCGATATTTA
TTGTAGGTCACGTAACAAAAGAAGGATCCATCGCGGGGCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATACTTT
GAAGGAGAACGTCACCATACTTTCCGAATCTTACGGGCTGTAAAAAACCGCTTTGGTTCTACAAACGAAATGGGTATTTT
TGAAATGCGGGAAGAAGGGCTTACTGAGGTACTGAATCCTTCAGAGATCTTCTTAGAAGAGCGTTCGGCGGGATCTGCGG
GGTCGAGCATAACGGCTTCTATGGAAGGTACAAGGCCGATTCTTGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGCATGGCAACAGGGATAGATCATAACAGGGTGTCACTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCATATTTGAAGGTAGCGGGCGGCGTCAAACTTGATGAACCTGCGATTGATCTTGCTA
TAGCGGTCAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGCCGGGTATCAAGAATTGAACAGCGTGTAAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
TGCGGCAAATCTGGATGGATGGACGAAACCAAAAGGGATTGAGGTCATCGGAGTAGCAAATGTTGCAGAGGCACTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

98.472

100

0.985
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.576

98.908

0.629