Detailed information NTG0474474

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: H7F27_RS02130
• Genome accession: NZ_CP060193
• Coordinates: 429940..431085 (-)
• Length: 381 a.a.
• NCBI ID: WP_185848498.1
• Uniprot ID: A0A7G7UEF1
• Organism: Bacillus sp. PAMC26543
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	428550..429700	429940..431085	flank	240

Gene organization within MGE regions

Location: 428550..431085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7F27_RS02120 (H7F27_02120)	-	428550..429700 (+)	1151	WP_185848496.1	IS3 family transposase	-
H7F27_RS02125 (H7F27_02125)	phrF	429837..429956 (-)	120	WP_185848497.1	PhrC/PhrF family phosphatase-inhibitory pheromone	Regulator
H7F27_RS02130 (H7F27_02130)	rapF	429940..431085 (-)	1146	WP_185848498.1	tetratricopeptide repeat protein	Regulator

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45777.26 Da
• Isoelectric Point: 5.1582

>NTDB_id=474474 H7F27_RS02130 WP_185848498.1 429940..431085(-) (rapF) [Bacillus sp. PAMC26543]
MTSVISSSSVGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLENMRIEEQPR
LSDLLLEIDKKQARLTGVLDYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHTAYNIRLLQCHSLFATNFLDLKQYEDAISHFKKAYTMAESEKQPQLMGRTLYNIGLCKNSQEQYES
AITYFKRAISVFEEANILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGIAYSKEAEDIIYLSEFEFLKSLYLSGPDEEAI
QKFFDFLESKMMYADLEDFALDVAKYYHGRENFQKASAYFLKVEQVRQLIQGGVRLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=474474 H7F27_RS02130 WP_185848498.1 429940..431085(-) (rapF) [Bacillus sp. PAMC26543]
GTGACAAGTGTCATATCTTCTTCTTCCGTTGGAGAAAAGATTAATGAATGGTATATGTACATACGCCGATTCAGCATACC
GGATGCGGAATATTTACGAAGAGAAATCAAACAAGAGCTGGATCAAATGGAAGAAGACCAGGATCTTCATTTGTATTATT
CACTGATGGAATTCCGGCACAATCTGATGCTTGAGTATCTTGAACCGTTAGAAAACATGCGGATAGAGGAACAGCCGCGA
CTGTCCGATTTGCTGCTTGAAATCGATAAAAAACAGGCTCGTTTAACCGGAGTCCTTGATTATTACTTTAATTTTTTCAG
GGGCATGTACGAACTGGACCAGCGGGAGTACTTGTCTGCTATTAAATTTTTCAAAAAAGCCGAAAGCAAGCTGATTTTTG
TAAAGGATCGAATAGAAAAAGCTGAGTTTTTCTTTAAAATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCC
ATGGACTATGCACGGCAGGCATATGAAATATATAAAGAGCACACGGCATATAATATTAGATTGCTTCAATGTCATTCTTT
GTTTGCTACCAATTTCTTAGATCTCAAGCAATATGAGGACGCCATATCACATTTCAAAAAAGCTTATACAATGGCTGAAT
CCGAAAAACAGCCCCAATTAATGGGAAGAACACTGTATAACATCGGACTTTGCAAAAACAGCCAAGAACAGTATGAGTCA
GCAATCACATATTTCAAAAGAGCCATATCTGTTTTTGAAGAAGCTAACATTCTTCCTTCTCTGCCTCAAGCTTATTTTTT
GATTACACAAATTCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAAGGAATTGCTTATTCTAAAG
AGGCTGAAGATATCATTTACTTATCGGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAGGCAATT
CAGAAATTCTTCGATTTTCTCGAAAGTAAAATGATGTATGCTGATCTTGAAGATTTTGCGCTTGATGTGGCAAAATATTA
TCATGGACGTGAAAATTTCCAAAAAGCTTCCGCGTATTTTCTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGATTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-207)

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

3D structure

• AlphaFold DB: A0A7G7UEF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	93.176	100	0.932
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572