Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GWQ54_RS19965 Genome accession   NZ_CP070373
Coordinates   4548994..4550676 (+) Length   560 a.a.
NCBI ID   WP_240361777.1    Uniprot ID   -
Organism   Streptomyces sp. MBT27     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4543994..4555676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWQ54_RS19940 - 4544515..4545744 (+) 1230 WP_240361775.1 hypothetical protein -
  GWQ54_RS19945 - 4545922..4546095 (+) 174 WP_167161177.1 hypothetical protein -
  GWQ54_RS19950 - 4546379..4546936 (-) 558 WP_229860864.1 PadR family transcriptional regulator -
  GWQ54_RS19955 - 4547111..4547929 (-) 819 WP_167161176.1 A24 family peptidase -
  GWQ54_RS19960 - 4548213..4548557 (-) 345 WP_240361776.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GWQ54_RS19965 pilF 4548994..4550676 (+) 1683 WP_240361777.1 GspE/PulE family protein Machinery gene
  GWQ54_RS19970 - 4550754..4551965 (+) 1212 WP_167161174.1 type II secretion system F family protein -
  GWQ54_RS19975 - 4551962..4552390 (+) 429 WP_167161173.1 type II secretion system protein -
  GWQ54_RS19980 - 4552387..4552911 (+) 525 WP_167161172.1 type II secretion system protein -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 60618.23 Da        Isoelectric Point: 5.1262

>NTDB_id=474062 GWQ54_RS19965 WP_240361777.1 4548994..4550676(+) (pilF) [Streptomyces sp. MBT27]
MGQKQPRIGELLVQRGLLTPAQVDEVLLQQSASGKRLGALLVELGMVEERSLIEALSVQLGVPVADLRQSEPDPEAAARV
PESLARKLGVVAVRRVEGTLLIAAGDPGDPRVLEEVGRAVAPDRVQLAIAVPADIRRSIDSAYRALAGVSSLVEAFEATE
TGRNADSGTRVVSEDAPVVHLVNLMLTQGLRDRASDIHIEPHADRVRVRYRIDGALHDVLSLPENMGPSVVSRIKIMANM
NIVDRRRPQDGQISTEIDGRAVDIRVATSPTIWGEKAVLRLLDSNRTLFRLAELGMPAATYERFSSLLRSPYGMVACGGP
TGSGKTTTLYATLGELNQSERNITTIEDPVEYVFPSVNQIQINDQAGITFATGLRSILRQDPDVILVGEIRDAETARIAV
ESALTGHFVLSSIHAADACSALHRFTDMGIEPFLITSTVAGVVSQRLVRRICDQCREPYRPGVDELAFFAKAGGSTDTGF
WQGRGCNFCSRTGYQERIGVFELLVMSDAVKGLLDEKAPIGSLRKAAREQQMDSLLDGGVRLVEEGITTIAEIMSSVYVV

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=474062 GWQ54_RS19965 WP_240361777.1 4548994..4550676(+) (pilF) [Streptomyces sp. MBT27]
GTGGGGCAGAAGCAGCCGCGTATTGGGGAGTTGCTGGTGCAGCGCGGGCTGCTCACCCCGGCGCAGGTGGACGAGGTGCT
CCTGCAGCAGTCGGCGTCGGGCAAGCGGCTCGGCGCCCTGCTGGTCGAACTCGGCATGGTCGAGGAGCGGTCACTGATCG
AGGCGCTGTCGGTTCAACTCGGCGTCCCCGTCGCCGATCTGCGCCAGAGCGAACCCGATCCCGAGGCTGCCGCCCGGGTG
CCGGAGTCGCTGGCCCGCAAGCTGGGCGTCGTCGCGGTGCGGCGGGTTGAGGGAACGCTGCTGATCGCCGCGGGCGACCC
GGGCGACCCGCGCGTGCTGGAGGAAGTCGGCCGGGCCGTCGCGCCGGATCGGGTGCAGTTGGCGATCGCGGTCCCCGCGG
ACATCCGGCGCTCGATCGACAGCGCCTACCGGGCGTTGGCCGGTGTCAGCAGCCTGGTCGAGGCCTTCGAGGCGACCGAG
ACCGGCCGTAACGCCGACTCCGGGACCAGGGTGGTGAGCGAGGACGCGCCGGTCGTGCACCTGGTCAACCTCATGCTGAC
CCAGGGCCTGCGCGACCGGGCCTCGGACATCCACATCGAACCGCACGCCGACCGGGTCCGGGTCCGGTACCGCATCGACG
GCGCGCTGCACGACGTGCTGAGCCTGCCGGAGAACATGGGCCCGTCCGTGGTCAGCCGGATCAAGATCATGGCCAACATG
AACATCGTCGACCGACGGCGCCCGCAGGACGGGCAGATCTCCACCGAGATCGACGGCAGGGCCGTCGACATCCGGGTCGC
CACCAGCCCCACCATCTGGGGCGAGAAGGCGGTCCTGCGGCTGCTCGACAGCAACCGCACACTGTTCCGCCTCGCCGAGC
TCGGCATGCCCGCCGCGACCTACGAACGGTTCTCCTCCTTGCTGCGCTCCCCGTACGGCATGGTCGCCTGCGGTGGGCCG
ACGGGCAGCGGCAAGACGACCACCCTGTACGCCACGCTCGGCGAGCTGAACCAGAGCGAGCGCAACATCACCACCATCGA
AGACCCGGTGGAGTACGTGTTCCCTTCGGTCAACCAGATCCAGATCAACGACCAGGCGGGCATCACCTTCGCGACCGGGT
TGCGCTCGATCCTGCGGCAGGACCCCGACGTGATCCTGGTCGGCGAGATCCGGGACGCCGAGACGGCCCGGATCGCGGTC
GAGTCCGCCCTCACCGGCCACTTCGTGCTGTCGTCCATCCACGCCGCCGACGCCTGTTCCGCGCTGCACCGGTTCACCGA
CATGGGCATCGAGCCGTTCCTGATCACCTCGACGGTCGCCGGTGTGGTCAGCCAGCGACTGGTACGGCGGATCTGCGACC
AGTGCCGCGAGCCCTACCGGCCGGGCGTGGACGAACTGGCCTTCTTCGCCAAGGCCGGCGGGTCCACCGACACCGGGTTC
TGGCAGGGCCGGGGCTGCAACTTCTGCTCCCGAACGGGCTATCAGGAGCGGATCGGGGTCTTCGAACTGCTCGTGATGAG
CGACGCCGTCAAGGGGTTGCTCGACGAGAAGGCGCCGATCGGCAGCCTGCGCAAGGCCGCCCGCGAGCAGCAGATGGACA
GCCTCCTGGACGGAGGCGTCCGGCTGGTGGAAGAGGGGATCACGACCATCGCCGAGATCATGTCCAGCGTCTACGTGGTC
TAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-145)

Type II/IV secretion system protein (T2SSE)

(184-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.087

98.929

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.5

100

0.375
  pilB Legionella pneumophila strain ERS1305867

36.879

100

0.371