Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H5404_RS22580 Genome accession   NZ_CP060088
Coordinates   3130048..3131064 (-) Length   338 a.a.
NCBI ID   WP_140219923.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain TJA114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3125048..3136064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5404_RS22555 (H5404_22555) aroK 3125908..3126426 (-) 519 WP_005381319.1 shikimate kinase AroK -
  H5404_RS22560 (H5404_22560) pilQ 3126615..3128363 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  H5404_RS22565 (H5404_22565) pilP 3128395..3128910 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  H5404_RS22570 (H5404_22570) pilO 3128900..3129493 (-) 594 WP_140219921.1 type 4a pilus biogenesis protein PilO Machinery gene
  H5404_RS22575 (H5404_22575) pilN 3129486..3130064 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  H5404_RS22580 (H5404_22580) pilM 3130048..3131064 (-) 1017 WP_140219923.1 type IV pilus biogenesis protein PilM Machinery gene
  H5404_RS22585 (H5404_22585) - 3131207..3133777 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  H5404_RS22590 (H5404_22590) oxyR 3133833..3134741 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  H5404_RS22595 (H5404_22595) - 3134912..3135640 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37832.42 Da        Isoelectric Point: 6.8323

>NTDB_id=473910 H5404_RS22580 WP_140219923.1 3130048..3131064(-) (pilM) [Vibrio parahaemolyticus strain TJA114]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRIEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMVTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=473910 H5404_RS22580 WP_140219923.1 3130048..3131064(-) (pilM) [Vibrio parahaemolyticus strain TJA114]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAATTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAATTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCAAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGTGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

49.107

99.408

0.488