Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H5404_RS22570 Genome accession   NZ_CP060088
Coordinates   3128900..3129493 (-) Length   197 a.a.
NCBI ID   WP_140219921.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain TJA114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123900..3134493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5404_RS22550 (H5404_22550) aroB 3124776..3125876 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  H5404_RS22555 (H5404_22555) aroK 3125908..3126426 (-) 519 WP_005381319.1 shikimate kinase AroK -
  H5404_RS22560 (H5404_22560) pilQ 3126615..3128363 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  H5404_RS22565 (H5404_22565) pilP 3128395..3128910 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  H5404_RS22570 (H5404_22570) pilO 3128900..3129493 (-) 594 WP_140219921.1 type 4a pilus biogenesis protein PilO Machinery gene
  H5404_RS22575 (H5404_22575) pilN 3129486..3130064 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  H5404_RS22580 (H5404_22580) pilM 3130048..3131064 (-) 1017 WP_140219923.1 type IV pilus biogenesis protein PilM Machinery gene
  H5404_RS22585 (H5404_22585) - 3131207..3133777 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23105.66 Da        Isoelectric Point: 4.8289

>NTDB_id=473908 H5404_RS22570 WP_140219921.1 3128900..3129493(-) (pilO) [Vibrio parahaemolyticus strain TJA114]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLEE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=473908 H5404_RS22570 WP_140219921.1 3128900..3129493(-) (pilO) [Vibrio parahaemolyticus strain TJA114]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGAAGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.684

96.447

0.904
  pilO Vibrio cholerae strain A1552

72.081

100

0.721