Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   H5404_RS22575 Genome accession   NZ_CP060088
Coordinates   3129486..3130064 (-) Length   192 a.a.
NCBI ID   WP_005480484.1    Uniprot ID   Q87L63
Organism   Vibrio parahaemolyticus strain TJA114     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3124486..3135064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5404_RS22550 (H5404_22550) aroB 3124776..3125876 (-) 1101 WP_005480443.1 3-dehydroquinate synthase -
  H5404_RS22555 (H5404_22555) aroK 3125908..3126426 (-) 519 WP_005381319.1 shikimate kinase AroK -
  H5404_RS22560 (H5404_22560) pilQ 3126615..3128363 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  H5404_RS22565 (H5404_22565) pilP 3128395..3128910 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  H5404_RS22570 (H5404_22570) pilO 3128900..3129493 (-) 594 WP_140219921.1 type 4a pilus biogenesis protein PilO Machinery gene
  H5404_RS22575 (H5404_22575) pilN 3129486..3130064 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  H5404_RS22580 (H5404_22580) pilM 3130048..3131064 (-) 1017 WP_140219923.1 type IV pilus biogenesis protein PilM Machinery gene
  H5404_RS22585 (H5404_22585) - 3131207..3133777 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  H5404_RS22590 (H5404_22590) oxyR 3133833..3134741 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.91 Da        Isoelectric Point: 6.6554

>NTDB_id=473909 H5404_RS22575 WP_005480484.1 3129486..3130064(-) (pilN) [Vibrio parahaemolyticus strain TJA114]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEQKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=473909 H5404_RS22575 WP_005480484.1 3129486..3130064(-) (pilN) [Vibrio parahaemolyticus strain TJA114]
GTGCTTTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTGGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGTGTTGGCAAATATTATGAGTATCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCTCGGCTAGCAACCATGCTCGATAACATGGAGCGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTCGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTTATGTTTAACACGGTAGAAGAGCAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594