Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   H5J27_RS27585 Genome accession   NZ_CP059995
Coordinates   5797999..5799591 (+) Length   530 a.a.
NCBI ID   WP_039028118.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY3045     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5792999..5804591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS27565 (H5J27_27565) pgeF 5794749..5795477 (-) 729 WP_033972914.1 peptidoglycan editing factor PgeF -
  H5J27_RS27570 (H5J27_27570) rluD 5795474..5796436 (-) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  H5J27_RS27575 (H5J27_27575) - 5796582..5797607 (+) 1026 WP_042168497.1 outer membrane protein assembly factor BamD -
  H5J27_RS27580 (H5J27_27580) - 5797764..5798009 (+) 246 WP_003102591.1 PP0621 family protein -
  H5J27_RS27585 (H5J27_27585) pilS 5797999..5799591 (+) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  H5J27_RS27590 (H5J27_27590) pilR 5799606..5800943 (+) 1338 WP_042168494.1 two-component system response regulator PilR Regulator
  H5J27_RS27595 (H5J27_27595) thiO 5800989..5802083 (-) 1095 WP_033972971.1 glycine oxidase ThiO -
  H5J27_RS27600 (H5J27_27600) - 5802218..5802727 (+) 510 WP_039028119.1 Tfp pilus assembly protein FimT/FimU -
  H5J27_RS27605 (H5J27_27605) - 5802833..5803339 (+) 507 WP_003134935.1 Tfp pilus assembly protein FimT/FimU -
  H5J27_RS27610 (H5J27_27610) pilV 5803330..5803887 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59116.28 Da        Isoelectric Point: 6.4439

>NTDB_id=473131 H5J27_RS27585 WP_039028118.1 5797999..5799591(+) (pilS) [Pseudomonas aeruginosa strain NY3045]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVLPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGADDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLVRDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=473131 H5J27_RS27585 WP_039028118.1 5797999..5799591(+) (pilS) [Pseudomonas aeruginosa strain NY3045]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
AGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAATATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCAGCGGCCAGCCTCG
GGCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAAGAACTCAACGCATTGATCCTGCAGCGTATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTACTCAGGCAGGACGACGTACAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCACCGACGCTCAAGGTGCT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCCGAGGAGCTGGACGCCCCGGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAT
AGCGAACTGCACCTGCAACTCGGTGCCGACGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGTGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGATAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.057

100

0.991