Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   R2846_RS01085 Genome accession   NC_017452
Coordinates   210435..210974 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae R2846     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 205435..215974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2846_RS01055 (R2846_0208) fdx 205838..206179 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  R2846_RS01060 (R2846_0209) iscX 206179..206373 (+) 195 WP_014550441.1 Fe-S cluster assembly protein IscX -
  R2846_RS01065 (R2846_0210) - 206424..207038 (-) 615 WP_014550442.1 YfgM family protein -
  R2846_RS01070 (R2846_0211) hisS 207056..208327 (-) 1272 WP_014550443.1 histidine--tRNA ligase -
  R2846_RS01075 (R2846_0212) ispG 208337..209443 (-) 1107 WP_014550444.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  R2846_RS01080 (R2846_0213) - 209452..210363 (-) 912 WP_014550445.1 helix-turn-helix domain-containing protein -
  R2846_RS01085 (R2846_0214) pilF/pilF2 210435..210974 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  R2846_RS01090 (R2846_0215) - 211075..212226 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  R2846_RS01095 (R2846_0216) - 212473..213351 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  R2846_RS01100 (R2846_0217) - 213716..214597 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  R2846_RS01105 (R2846_0218) - 214597..215517 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=47290 R2846_RS01085 WP_005654843.1 210435..210974(-) (pilF/pilF2) [Haemophilus influenzae R2846]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=47290 R2846_RS01085 WP_005654843.1 210435..210974(-) (pilF/pilF2) [Haemophilus influenzae R2846]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment