Detailed information NTG0472734

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H5A23_RS14315
• Genome accession: NZ_CP059963
• Coordinates: 3260857..3261702 (-)
• Length: 281 a.a.
• NCBI ID: WP_119871904.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain IPO:3649 NAK:375
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3255857..3266702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5A23_RS14290 (H5A23_14290)	-	3256059..3256829 (-)	771	WP_119871907.1	ABC transporter ATP-binding protein	-
H5A23_RS14295 (H5A23_14295)	-	3256831..3257868 (-)	1038	WP_119871906.1	FecCD family ABC transporter permease	-
H5A23_RS14300 (H5A23_14300)	-	3257865..3258929 (-)	1065	WP_119871905.1	ABC transporter substrate-binding protein	-
H5A23_RS14305 (H5A23_14305)	-	3259239..3260264 (+)	1026	WP_014916091.1	LLM class flavin-dependent oxidoreductase	-
H5A23_RS14310 (H5A23_14310)	ypdK	3260622..3260696 (+)	75	WP_099606229.1	membrane protein YpdK	-
H5A23_RS14315 (H5A23_14315)	pilD	3260857..3261702 (-)	846	WP_119871904.1	prepilin peptidase	Machinery gene
H5A23_RS14320 (H5A23_14320)	-	3261811..3262557 (-)	747	WP_119871903.1	type II secretion system protein N	-
H5A23_RS14325 (H5A23_14325)	gspM	3262547..3263041 (-)	495	WP_119871902.1	type II secretion system protein GspM	-
H5A23_RS14330 (H5A23_14330)	gspL	3263038..3264315 (-)	1278	WP_119871901.1	type II secretion system protein GspL	-
H5A23_RS14335 (H5A23_14335)	gspK	3264312..3265298 (-)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
H5A23_RS14340 (H5A23_14340)	gspJ	3265295..3265945 (-)	651	WP_119871900.1	type II secretion system minor pseudopilin GspJ	-
H5A23_RS14345 (H5A23_14345)	gspI	3265945..3266310 (-)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31466.46 Da
• Isoelectric Point: 6.4557

>NTDB_id=472734 H5A23_RS14315 WP_119871904.1 3260857..3261702(-) (pilD) [Pectobacterium brasiliense strain IPO:3649 NAK:375]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSDPDKRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLNDAVVGAMAGYLSLWLLYWVFKYVTGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=472734 H5A23_RS14315 WP_119871904.1 3260857..3261702(-) (pilD) [Pectobacterium brasiliense strain IPO:3649 NAK:375]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCATGGTGGTTTGGATCGCTGGGCGTGCTGGGATTGATTGTTGG
CAGTTTTTTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGCCGATTCCGATCCTGATAAGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCACACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAATGATGCCGTCGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGTAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.185	96.441	0.484
pilD	Neisseria gonorrhoeae MS11	47.126	92.883	0.438
pilD	Vibrio campbellii strain DS40M4	44.981	95.73	0.431
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402