Detailed information NTG0472570

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: H5A38_RS06790
• Genome accession: NZ_CP059956
• Coordinates: 1533009..1533854 (+)
• Length: 281 a.a.
• NCBI ID: WP_205557569.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain IPO:4132 NAK:239
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1528009..1538854

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5A38_RS06760 (H5A38_06760)	gspI	1528392..1528757 (+)	366	WP_039273738.1	type II secretion system minor pseudopilin GspI	-
H5A38_RS06765 (H5A38_06765)	gspJ	1528757..1529407 (+)	651	WP_104212426.1	type II secretion system minor pseudopilin GspJ	-
H5A38_RS06770 (H5A38_06770)	gspK	1529404..1530390 (+)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
H5A38_RS06775 (H5A38_06775)	gspL	1530387..1531673 (+)	1287	WP_104212427.1	type II secretion system protein GspL	-
H5A38_RS06780 (H5A38_06780)	gspM	1531670..1532164 (+)	495	WP_104212428.1	type II secretion system protein GspM	-
H5A38_RS06785 (H5A38_06785)	-	1532154..1532900 (+)	747	WP_205557567.1	type II secretion system protein N	-
H5A38_RS06790 (H5A38_06790)	pilD	1533009..1533854 (+)	846	WP_205557569.1	prepilin peptidase	Machinery gene
H5A38_RS06795 (H5A38_06795)	ypdK	1534015..1534089 (-)	75	WP_099606229.1	membrane protein YpdK	-
H5A38_RS06800 (H5A38_06800)	-	1534447..1535472 (-)	1026	WP_205557571.1	LLM class flavin-dependent oxidoreductase	-
H5A38_RS06805 (H5A38_06805)	-	1535782..1536846 (+)	1065	WP_040043676.1	ABC transporter substrate-binding protein	-
H5A38_RS06810 (H5A38_06810)	-	1536843..1537880 (+)	1038	WP_080724594.1	FecCD family ABC transporter permease	-
H5A38_RS06815 (H5A38_06815)	-	1537882..1538652 (+)	771	WP_205557573.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31483.51 Da
• Isoelectric Point: 5.7656

>NTDB_id=472570 H5A38_RS06790 WP_205557569.1 1533009..1533854(+) (pilD) [Pectobacterium brasiliense strain IPO:4132 NAK:239]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMVDSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFNWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSETFVPLIDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=472570 H5A38_RS06790 WP_205557569.1 1533009..1533854(+) (pilD) [Pectobacterium brasiliense strain IPO:4132 NAK:239]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTTATTTACCGCCTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGATATCGAGCTTGAAACGG
GCATGGTTGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCACCTTCGTCGTGCCCGCACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAACTGGTTGTGGTTGCGTGGCCGCTCCCGCTGCTGTCATCAATCGGTGTCTGT
GCAATATCCGCTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTACCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGCTGCTGTTTAATCTGTCGGAGACCTTTGTCCCATTGATTGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTACTCTATTGGGTATTCAAATACGCAACGGGAAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTCGTGACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.815	96.441	0.48
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Vibrio campbellii strain DS40M4	44.61	95.73	0.427
pilD	Acinetobacter baumannii D1279779	41.026	97.153	0.399
pilD	Acinetobacter nosocomialis M2	40.659	97.153	0.395