Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   H4K32_RS15950 Genome accession   NZ_CP059855
Coordinates   3070459..3071259 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain Pm9     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 3065459..3076259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4K32_RS15930 - 3065581..3066426 (+) 846 WP_015388017.1 TlyA family RNA methyltransferase -
  H4K32_RS15935 ahrC 3066523..3066972 (+) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  H4K32_RS15940 recN 3067004..3068734 (+) 1731 WP_003153174.1 DNA repair protein RecN Machinery gene
  H4K32_RS15945 spoIVB 3068898..3070178 (+) 1281 WP_007408360.1 SpoIVB peptidase -
  H4K32_RS15950 spo0A 3070459..3071259 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  H4K32_RS15955 alr 3071408..3072589 (-) 1182 WP_025852858.1 alanine racemase -
  H4K32_RS15960 - 3072764..3072976 (-) 213 WP_014305392.1 spore germination protein -
  H4K32_RS15965 - 3073105..3073317 (-) 213 WP_003153183.1 spore germination protein -
  H4K32_RS15970 - 3073443..3074561 (-) 1119 WP_182275267.1 NADH-dependent flavin oxidoreductase -
  H4K32_RS15975 - 3074655..3074792 (-) 138 WP_014470646.1 hypothetical protein -
  H4K32_RS15980 - 3075083..3075715 (-) 633 WP_014418348.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=472324 H4K32_RS15950 WP_003153177.1 3070459..3071259(+) (spo0A) [Bacillus velezensis strain Pm9]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=472324 H4K32_RS15950 WP_003153177.1 3070459..3071259(+) (spo0A) [Bacillus velezensis strain Pm9]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCCC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97