Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   H2513_RS06160 Genome accession   NZ_CP059704
Coordinates   1350456..1351001 (-) Length   181 a.a.
NCBI ID   WP_059245747.1    Uniprot ID   -
Organism   Pasteurella multocida strain RCAD0730     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1345456..1356001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2513_RS06135 (H2513_06115) - 1345666..1346103 (-) 438 WP_010907449.1 isoprenylcysteine carboxylmethyltransferase family protein -
  H2513_RS06140 (H2513_06120) - 1346316..1346927 (-) 612 WP_005725137.1 YfgM family protein -
  H2513_RS06145 (H2513_06125) hisS 1346946..1348217 (-) 1272 WP_010907448.1 histidine--tRNA ligase -
  H2513_RS06150 (H2513_06130) ispG 1348247..1349350 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H2513_RS06155 (H2513_06135) - 1349377..1350333 (-) 957 WP_059245748.1 RodZ family helix-turn-helix domain-containing protein -
  H2513_RS06160 (H2513_06140) pilF/pilF2 1350456..1351001 (-) 546 WP_059245747.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H2513_RS06165 (H2513_06145) - 1351041..1352225 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  H2513_RS06170 (H2513_06150) - 1352406..1353659 (-) 1254 WP_005755688.1 AI-2E family transporter -
  H2513_RS06175 (H2513_06155) glnB 1353660..1353998 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  H2513_RS06180 (H2513_06160) mog 1354043..1354621 (-) 579 WP_005737641.1 molybdopterin adenylyltransferase -
  H2513_RS06185 (H2513_06165) - 1354647..1355576 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20855.42 Da        Isoelectric Point: 8.1524

>NTDB_id=471471 H2513_RS06160 WP_059245747.1 1350456..1351001(-) (pilF/pilF2) [Pasteurella multocida strain RCAD0730]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=471471 H2513_RS06160 WP_059245747.1 1350456..1351001(-) (pilF/pilF2) [Pasteurella multocida strain RCAD0730]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459