Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H3V22_RS02715 Genome accession   NZ_CP059679
Coordinates   546957..547853 (-) Length   298 a.a.
NCBI ID   WP_182093962.1    Uniprot ID   -
Organism   Mammaliicoccus lentus strain H29     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541957..552853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3V22_RS02675 (H3V22_02675) - 542405..542923 (-) 519 WP_064205405.1 shikimate kinase -
  H3V22_RS02680 (H3V22_02680) - 543189..544361 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  H3V22_RS02685 (H3V22_02685) - 544579..544887 (-) 309 WP_239747373.1 hypothetical protein -
  H3V22_RS02690 (H3V22_02690) comGE 544901..545173 (-) 273 WP_228082628.1 competence type IV pilus minor pilin ComGE -
  H3V22_RS02695 (H3V22_02695) comGD 545160..545588 (-) 429 WP_017000239.1 competence type IV pilus minor pilin ComGD -
  H3V22_RS02700 (H3V22_02700) comGC 545588..545905 (-) 318 WP_070045385.1 competence type IV pilus major pilin ComGC Machinery gene
  H3V22_RS02705 (H3V22_02705) - 545923..546648 (-) 726 WP_182093960.1 type II secretion system F family protein -
  H3V22_RS02710 (H3V22_02710) - 546658..546957 (-) 300 WP_182093961.1 hypothetical protein -
  H3V22_RS02715 (H3V22_02715) comGA 546957..547853 (-) 897 WP_182093962.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  H3V22_RS02720 (H3V22_02720) - 548006..548254 (+) 249 WP_017000234.1 DUF2626 family protein -
  H3V22_RS02725 (H3V22_02725) - 548319..548951 (-) 633 WP_017000233.1 MBL fold metallo-hydrolase -
  H3V22_RS02730 (H3V22_02730) - 549049..549288 (+) 240 WP_017000232.1 DUF2759 domain-containing protein -
  H3V22_RS02735 (H3V22_02735) - 549317..549631 (-) 315 WP_017000231.1 MTH1187 family thiamine-binding protein -
  H3V22_RS02740 (H3V22_02740) - 549631..550602 (-) 972 WP_103268827.1 ROK family glucokinase -
  H3V22_RS02745 (H3V22_02745) - 550599..550790 (-) 192 WP_017000229.1 YqgQ family protein -
  H3V22_RS02750 (H3V22_02750) - 550793..552241 (-) 1449 WP_182093963.1 rhomboid family intramembrane serine protease -
  H3V22_RS02755 (H3V22_02755) - 552242..552796 (-) 555 WP_064204534.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34520.75 Da        Isoelectric Point: 6.1644

>NTDB_id=471293 H3V22_RS02715 WP_182093962.1 546957..547853(-) (comGA) [Mammaliicoccus lentus strain H29]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGENTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKMIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCYPDVVMIGEIRDEETAKQVIQASLSGHLILSTMRAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=471293 H3V22_RS02715 WP_182093962.1 546957..547853(-) (comGA) [Mammaliicoccus lentus strain H29]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACTGACATTCATTTTATTCCAGTGAAAGA
GGATCGAGTCAAAGTTCAGTTGAGGATAAGTGGTGAGAATACGATTTATGATTGTGATATTAATAACGCTTTATATAACC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATGATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTTATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCGTGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.331

100

0.51
  comGA Staphylococcus aureus N315

50.331

100

0.51