Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ToN1_RS10900 Genome accession   NZ_CP059560
Coordinates   2384839..2386284 (+) Length   481 a.a.
NCBI ID   WP_169208841.1    Uniprot ID   -
Organism   Aromatoleum petrolei strain ToN1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379839..2391284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ToN1_RS10870 (ToN1_22290) thiL 2380580..2381539 (+) 960 WP_169208216.1 thiamine-phosphate kinase -
  ToN1_RS10875 (ToN1_22300) - 2381561..2382040 (+) 480 WP_210148091.1 phosphatidylglycerophosphatase A -
  ToN1_RS10880 (ToN1_22310) - 2382045..2382524 (+) 480 WP_169208845.1 nicotinamide-nucleotide amidohydrolase family protein -
  ToN1_RS10885 (ToN1_22320) recA 2382610..2383644 (+) 1035 WP_169208844.1 recombinase RecA Machinery gene
  ToN1_RS10890 (ToN1_22330) recX 2383662..2384111 (+) 450 WP_169208843.1 recombination regulator RecX -
  ToN1_RS10895 (ToN1_22340) lptB 2384098..2384820 (+) 723 WP_169208842.1 LPS export ABC transporter ATP-binding protein -
  ToN1_RS10900 (ToN1_22350) rpoN/rpoN1 2384839..2386284 (+) 1446 WP_169208841.1 RNA polymerase factor sigma-54 Machinery gene
  ToN1_RS10905 (ToN1_22360) raiA 2386314..2386640 (+) 327 WP_169208840.1 ribosome-associated translation inhibitor RaiA -
  ToN1_RS10910 (ToN1_22370) ptsN 2386777..2387262 (+) 486 WP_169208839.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ToN1_RS10915 (ToN1_22380) hprK 2387231..2388172 (+) 942 WP_169208838.1 HPr(Ser) kinase/phosphatase -
  ToN1_RS10920 (ToN1_22390) ilvA 2388260..2389780 (+) 1521 WP_169208837.1 threonine ammonia-lyase, biosynthetic -
  ToN1_RS10925 (ToN1_22400) - 2389759..2390361 (-) 603 WP_169208847.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 53439.11 Da        Isoelectric Point: 4.7470

>NTDB_id=470628 ToN1_RS10900 WP_169208841.1 2384839..2386284(+) (rpoN/rpoN1) [Aromatoleum petrolei strain ToN1]
MKPSLQLKISQHLTLTPQLQQSIKLLQLSTLELNQELERFLLENPMLEREDGDGSDFAPGAAQPSGEAPDSGSQADTPTP
GEEGHGEAEPASFDEAGEWSSASSGSSNRDDDDDTDFQELQAAGTSLRDHLDQQVSLSPLSDRDRALVRFIIEALDEDGY
LNQSLEELLPLLPPELEYDLDDLSIALRHVQNLDPAGIGARNPQECLSLQLRAMPSDPTRDLALKIVDEHLDLLAERNFL
RIKRATGCDDDALRAAHALICRLDPHPGSQHSVQETRYILPDVVVRKIRNRWAVTLNPEAMPKLRINSLYASILQQNRGS
GGALTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRG
VFELKYFFGSHVATDTGGAASSTAIRALIRQLVEAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKA
I

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=470628 ToN1_RS10900 WP_169208841.1 2384839..2386284(+) (rpoN/rpoN1) [Aromatoleum petrolei strain ToN1]
ATGAAACCGTCCCTCCAGCTCAAGATCTCCCAGCACCTCACGCTGACGCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCGACGCTGGAGCTCAACCAGGAGCTGGAACGCTTCCTCCTCGAGAACCCCATGCTCGAGCGCGAGGACGGCGACG
GCAGCGACTTCGCCCCGGGTGCCGCGCAGCCCTCCGGCGAAGCGCCCGACTCCGGCAGCCAGGCCGACACCCCGACGCCC
GGCGAGGAGGGGCATGGCGAGGCCGAACCGGCGAGTTTCGACGAAGCCGGCGAATGGTCGAGCGCGAGCAGTGGTTCGTC
GAACCGCGACGATGACGACGACACCGACTTCCAGGAACTGCAGGCTGCCGGCACCAGCCTGCGCGATCACCTCGACCAGC
AGGTGAGCCTGTCGCCGCTGTCGGATCGCGACCGCGCGCTGGTGCGCTTCATCATCGAGGCGCTCGACGAGGACGGCTAC
CTCAACCAGTCGCTCGAGGAGCTGCTGCCGCTGCTGCCGCCGGAACTCGAATACGACCTCGACGACCTCTCGATCGCGTT
GCGCCACGTGCAGAACCTCGACCCGGCCGGCATCGGCGCGCGCAACCCGCAGGAATGCCTCAGCCTGCAACTCCGCGCAA
TGCCGTCGGACCCGACGCGTGACCTCGCGCTGAAGATCGTCGACGAGCACCTCGACCTGCTCGCTGAACGCAACTTTCTG
CGCATCAAGCGCGCCACCGGCTGCGACGACGACGCACTGCGCGCCGCGCACGCGCTGATCTGCAGGCTCGATCCGCATCC
GGGCTCGCAGCACTCGGTGCAGGAGACGCGCTACATCCTGCCGGATGTCGTCGTGCGCAAGATCCGCAACCGCTGGGCCG
TCACGCTCAACCCCGAGGCGATGCCCAAGCTGCGCATCAACTCACTGTACGCCAGCATCCTGCAACAGAACCGCGGATCG
GGCGGCGCGCTGACCAGCCAGCTGCAGGAAGCACGCTGGCTAATCAAGAACGTGCAACAGCGTTTCGACACCATCCTGCG
GGTTTCGCAGGCGATCGTTGACCAGCAGCGACAGTTCTTCGATTATGGTGATGTGGCGATGCGACCCCTGACGTTGAGGG
AGATCGCGGACCAGCTCGAACTGCACGAATCGACTGTTTCGCGGGTCACGACGCAGAAATTCATGGCCACACCGCGCGGC
GTGTTCGAACTGAAGTATTTTTTCGGCAGTCACGTTGCCACCGACACCGGTGGGGCGGCCTCTTCCACGGCGATTCGAGC
GCTGATTCGCCAGCTGGTGGAGGCCGAAGACAGGAAAAAGCCCCTGTCCGACGCGAAAATTGCCGAGTTGTTGGGCCAGC
AGGGTATCGTGGTGGCGCGCAGAACGATCGCGAAATACCGCGAGTCACTCAATATTCCGCCGGTCAGCCTGCGCAAAGCG
ATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(324-479)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-310)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.666

100

0.582