Detailed information NTG0470165

Bioinformatically predicted

Overview

• Name: degS
• Type: Regulator
• Locus tag: H2N74_RS17225
• Genome accession: NZ_CP059495
• Coordinates: 3526259..3527422 (-)
• Length: 387 a.a.
• NCBI ID: WP_007407484.1
• Uniprot ID: A3KLC1
• Organism: Bacillus velezensis strain JSRB 166
• Function: phosphorylation of DegU (predicted from homology) Competence regulation

Genomic Context

Location: 3521259..3532422

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2N74_RS17195 (H2N74_17195)	-	3521524..3521943 (-)	420	WP_014419009.1	TIGR03826 family flagellar region protein	-
H2N74_RS17200 (H2N74_17200)	comFC	3522017..3522706 (-)	690	WP_199449054.1	ComF family protein	Machinery gene
H2N74_RS17205 (H2N74_17205)	-	3522712..3522996 (-)	285	WP_003151389.1	late competence development ComFB family protein	-
H2N74_RS17210 (H2N74_17210)	comFA	3523054..3524439 (-)	1386	WP_095060187.1	DEAD/DEAH box helicase	Machinery gene
H2N74_RS17215 (H2N74_17215)	-	3524547..3525395 (-)	849	WP_003151387.1	DegV family protein	-
H2N74_RS17220 (H2N74_17220)	degU	3525493..3526182 (-)	690	WP_003219701.1	two-component system response regulator DegU	Regulator
H2N74_RS17225 (H2N74_17225)	degS	3526259..3527422 (-)	1164	WP_007407484.1	two-component sensor histidine kinase DegS	Regulator
H2N74_RS17230 (H2N74_17230)	-	3527645..3528292 (+)	648	WP_007407485.1	YigZ family protein	-
H2N74_RS17235 (H2N74_17235)	-	3528297..3529511 (+)	1215	WP_014419013.1	LCP family protein	-
H2N74_RS17240 (H2N74_17240)	-	3529601..3530677 (-)	1077	WP_003151382.1	glycosyltransferase family 4 protein	-
H2N74_RS17245 (H2N74_17245)	-	3530810..3532006 (-)	1197	WP_182072047.1	glycosyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 45014.78 Da
• Isoelectric Point: 6.8616

>NTDB_id=470165 H2N74_RS17225 WP_007407484.1 3526259..3527422(-) (degS) [Bacillus velezensis strain JSRB 166]
MNKSKMDSKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVHTRHARNRLSEVSR
NFHKFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLGLQEIIERSESLVSQITVVLNYLNQDLRQVGLLL
ADAQAKQDFGLRIIEAQEEERKRVSREIHDGPAQMLANVMMRSELIERIFRDRGTEDGFQEIRNLRQNVRNALYEVRRII
YDLRPMALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGDTENQRLAPQFEVALFRLAQEAVTNALKHSESEEITVKVEV
TADFVVLIIKDNGKGFDIKDAKQKKNKSFGLLGMKERVDLLEGTITIDSKIGLGTFIMIKVPLSLGL

Nucleotide

• Length: 1164 bp

>NTDB_id=470165 H2N74_RS17225 WP_007407484.1 3526259..3527422(-) (degS) [Bacillus velezensis strain JSRB 166]
ATGAATAAATCGAAGATGGACTCCAAAGTTTTGGATTCTATACTTATGAAAATGCTGAAAACCGTGGACGGGAGTAAGGA
CGAGGTTTTTCAAATCGGAGAGCAGTCGCGCCAGCAATATGAGCAGCTGGTTGAAGAACTCAAACAGATCAAACAGCAGG
TCTACGAAGTCATTGAGCTGGGCGACAAACTTGAAGTTCATACGCGGCACGCACGAAACCGCTTGTCTGAAGTAAGCAGA
AATTTCCATAAGTTCAGCGAAGAAGAAATTCGCAACGCCTATGAAAAGGCGCACAAACTTCAAGTGGAACTGACGATGAT
CCAACAGCGTGAAAAGCAGCTGCGGGAGCGCAGAGATGATTTAGAACGCCGGCTTTTAGGTCTTCAGGAAATCATTGAAC
GGTCAGAATCGTTGGTCAGCCAAATAACCGTCGTTCTGAATTACCTGAATCAAGACCTTCGCCAAGTAGGGCTTCTTCTT
GCCGATGCGCAGGCTAAACAAGATTTCGGTTTAAGAATCATTGAAGCCCAAGAGGAAGAAAGAAAAAGAGTTTCCAGAGA
AATTCATGACGGTCCCGCGCAAATGCTGGCAAATGTCATGATGAGATCTGAATTAATTGAACGGATCTTCCGCGACCGCG
GCACGGAGGACGGATTTCAGGAAATCAGAAATCTACGGCAGAATGTGCGGAATGCCCTTTACGAAGTCAGAAGGATTATA
TATGATTTAAGACCGATGGCATTGGACGATCTCGGGCTGATTCCGACACTGAGAAAATATCTTTATACAACTGAAGAATA
CAACGGAAAGGTGAAAATACATTTTCAGTGCATCGGAGACACGGAGAACCAAAGGCTTGCCCCGCAATTTGAAGTGGCGC
TTTTCCGTCTCGCTCAGGAAGCCGTCACAAATGCACTCAAGCATTCAGAATCAGAGGAAATTACAGTAAAAGTGGAAGTA
ACCGCTGATTTTGTCGTTCTCATCATTAAGGACAATGGAAAAGGCTTTGATATTAAGGATGCTAAACAAAAGAAAAACAA
GTCATTCGGCTTGCTGGGAATGAAAGAAAGAGTAGATCTGCTTGAAGGAACGATAACGATAGACTCTAAAATAGGTCTTG
GGACATTTATCATGATTAAGGTTCCGTTATCTCTCGGCCTATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (291-384)

• Sensor protein DegS (DegS) (12-170)

• Histidine kinase (HisKA_3) (180-248)

3D structure

• AlphaFold DB: A3KLC1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
degS	Bacillus subtilis subsp. subtilis str. 168	95.584	99.483	0.951