Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JSQ77_RS02140 Genome accession   NZ_CP069712
Coordinates   469625..470302 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EA13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 464625..475302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ77_RS02125 (JSQ77_02130) chiA 465992..468685 (+) 2694 WP_104696708.1 bifunctional chitinase/lysozyme -
  JSQ77_RS02130 (JSQ77_02135) bfd 468854..469048 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JSQ77_RS02135 (JSQ77_02140) bfr 469120..469596 (+) 477 WP_000675504.1 bacterioferritin -
  JSQ77_RS02140 (JSQ77_02145) pilD 469625..470302 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JSQ77_RS02145 (JSQ77_02150) gspM 470302..470763 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JSQ77_RS02150 (JSQ77_02155) gspL 470760..471923 (-) 1164 WP_112901553.1 type II secretion system protein GspL -
  JSQ77_RS02155 (JSQ77_02160) gspK 471938..472921 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JSQ77_RS02160 (JSQ77_02165) gspJ 472914..473501 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JSQ77_RS02165 (JSQ77_02170) gspI 473494..473871 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JSQ77_RS02170 (JSQ77_02175) gspH 473868..474377 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  JSQ77_RS02175 (JSQ77_02180) gspG 474385..474822 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=470074 JSQ77_RS02140 WP_000178161.1 469625..470302(-) (pilD) [Escherichia coli strain EA13]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=470074 JSQ77_RS02140 WP_000178161.1 469625..470302(-) (pilD) [Escherichia coli strain EA13]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378