Detailed information NTG0470062

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: H2R00_RS09260
• Genome accession: NZ_CP059494
• Coordinates: 1858510..1862202 (-)
• Length: 1230 a.a.
• NCBI ID: WP_154994078.1
• Uniprot ID: -
• Organism: Bacillus haynesii strain P19
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1853510..1867202

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2R00_RS09245	-	1853611..1853829 (+)	219	WP_016885923.1	spore germination protein	-
H2R00_RS09250	sbcC	1853874..1857266 (-)	3393	WP_182070896.1	exonuclease subunit SbcC	-
H2R00_RS09255	sbcD	1857263..1858444 (-)	1182	WP_182070897.1	exonuclease subunit SbcD	-
H2R00_RS09260	addA	1858510..1862202 (-)	3693	WP_154994078.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
H2R00_RS09265	addB	1862189..1865689 (-)	3501	WP_182070898.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
H2R00_RS09270	modB	1865798..1866454 (-)	657	WP_154994075.1	molybdate ABC transporter permease subunit	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141128.92 Da
• Isoelectric Point: 5.4189

>NTDB_id=470062 H2R00_RS09260 WP_154994078.1 1858510..1862202(-) (addA) [Bacillus haynesii strain P19]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEYPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQNDFSALHELVPAVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFTRTPEQHLESLREMKPVIQTLVQLVLEYGKRFAEAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIENQSGVPDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAVKAYLAAADGDERLSEKLRRFDGLLKKWRAFAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPPVIDEIRKHPARFQVSWLSAADLQAE
DVDRAGEERHGRLAQIQMGLPIDGSFEYEQQVRERLTWSYPYNDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSQEAF
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEMNELSSLLDRLVEKELLTEDQRAAIGEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=470062 H2R00_RS09260 WP_154994078.1 1858510..1862202(-) (addA) [Bacillus haynesii strain P19]
ATGGAGATTTCTAAACCGAAGGGCAGCACTTGGACTGATGACCAATGGAAGGCGATCGTCTCATCCGGCCGGGACATTTT
AGTCGCGGCAGCGGCCGGTTCCGGAAAAACGGCCGTGCTGGTTGAACGGATCATTCGGAAAATAACGGATCAGGAATACC
CAGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAATAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATATGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTAGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTCGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTTGTCAAGCGGGTCTACGAGTT
TTCCCGCTCGCATCCCGATCCTGAGGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACAAAAGTCG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAACAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCAGCCCTGCATGAGCTTGTGCCTGCTGTTTCGTTTCAAAGATTGAAGCCATGCAAAG
GAGATGAGTACGATCCTCGATTGGTTGATGAGGCGGCAGACCTTCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTACGCGAACTCCCGAGCAACACCTTGAGAGTCTGAGGGAAATGAAGCCGGTCATTCAGACACTCGTCCA
GCTCGTACTAGAGTACGGCAAGCGGTTTGCGGAAGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTCGAACACG
ACTGCCTTGCGATTTTATCCGGAAAAAACCAAGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTCGATGAATATCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACTTGTTTATGGTGGGCGATGTTAAGCAGTCGATTTACCGGTTCCGCCTCGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAAAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
AGCCGAATTGAAGCTCGGCGCGTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGTGCCGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAAATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGGAACATCCAGTACCGTGATATTGTCGTGCTTTT
ACGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGGATTCCCGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCATCCGTTCTCAGATCGCCTGTTGTGGGTCTTGACGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGGTGAAAGCATACCTTGCTGCAGCAGACGGCGACGAGAGGCTTAGCGAAAAGCTTCGCCGCTTTGACG
GCCTGCTCAAAAAGTGGAGAGCGTTTGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTACGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GTACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTCGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCTAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCCGGGTCTGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCGAAATCATACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGAGCGC
CGCCGGTGATTGATGAGATCCGTAAACATCCTGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTTCAAGCTGAG
GATGTTGATCGGGCCGGTGAAGAAAGACACGGCCGGCTCGCCCAAATTCAAATGGGGCTTCCGATCGACGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAACGGCTGACATGGTCATATCCATACAATGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGACAGAAGGAATATGAGGATGAATACGGCGACCGTTCGCTGATCCGCCCATCCCAGGAGGCTTTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACTGCGGCCGAACGGGGAACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAATGAATGAGCTCAGCAGTCTCCTTGACAGACTTGTTGAGAAAGAACTGTTAA
CAGAAGATCAGCGGGCAGCTATTGGCGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCGTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCGGATCTCGAGTCAGCCGA
TGAACCTGTATTGATACAGGGAATTATCGACTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCG
ACCGGATACACGGAAAATACCGGAACGGTTTTGAAGGAGCGGAACCGATTTTAAGAAAACGTTACGAAACACAGATTCAG
CTTTATGCACGAGCGGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCGCTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	72.114	100	0.721