Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H2R00_RS02200 Genome accession   NZ_CP059494
Coordinates   424685..425749 (+) Length   354 a.a.
NCBI ID   WP_154997143.1    Uniprot ID   -
Organism   Bacillus haynesii strain P19     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419685..430749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2R00_RS02160 - 420107..421216 (+) 1110 WP_182070383.1 hypothetical protein -
  H2R00_RS02165 - 421232..421546 (+) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  H2R00_RS02170 - 421606..421779 (-) 174 WP_003183486.1 DUF2759 domain-containing protein -
  H2R00_RS02175 - 421935..422573 (+) 639 WP_182070384.1 MBL fold metallo-hydrolase -
  H2R00_RS02180 - 422609..422854 (-) 246 WP_020452180.1 DUF2626 domain-containing protein -
  H2R00_RS02185 - 423065..423442 (+) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  H2R00_RS02195 - 423687..424526 (+) 840 WP_154997141.1 STAS domain-containing protein -
  H2R00_RS02200 comGA 424685..425749 (+) 1065 WP_154997143.1 competence type IV pilus ATPase ComGA Machinery gene
  H2R00_RS02205 comGB 425739..426773 (+) 1035 WP_154997145.1 competence type IV pilus assembly protein ComGB Machinery gene
  H2R00_RS02210 comGC 426787..427080 (+) 294 WP_043927885.1 competence type IV pilus major pilin ComGC Machinery gene
  H2R00_RS02215 comGD 427110..427523 (+) 414 WP_244958271.1 competence type IV pilus minor pilin ComGD -
  H2R00_RS02220 comGE 427507..427854 (+) 348 WP_182070386.1 competence type IV pilus minor pilin ComGE -
  H2R00_RS02225 comGF 427880..428251 (+) 372 WP_261763563.1 competence type IV pilus minor pilin ComGF -
  H2R00_RS02230 comGG 428263..428628 (+) 366 WP_154997614.1 competence type IV pilus minor pilin ComGG -
  H2R00_RS02235 - 428717..428899 (+) 183 WP_020452171.1 YqzE family protein -
  H2R00_RS02240 - 428929..429249 (-) 321 WP_076793488.1 YqzG/YhdC family protein -
  H2R00_RS02245 tapA 429526..430254 (+) 729 WP_182070387.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39695.25 Da        Isoelectric Point: 9.2682

>NTDB_id=470035 H2R00_RS02200 WP_154997143.1 424685..425749(+) (comGA) [Bacillus haynesii strain P19]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLELGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCKLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GGIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=470035 H2R00_RS02200 WP_154997143.1 424685..425749(+) (comGA) [Bacillus haynesii strain P19]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTGTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGACATTGGTGAAAGACGGCTGCCGCAAAGCGGGGCTTTGACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCCTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAACCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTGTTCACCGGTCCCACAGGCTCTGGGAAAACAACAACACTCTATTCTTTAATTGAATAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCTGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACTGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCTAAAGGTGCGATATACAGACTGCTCGAGCTTGGCGTTGATATGACAGAAATTGAACAAACATTGGTCGC
AATCAGCGCCCAGCGTCTTGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTGTATTGTAAATTGTCGAGAC
CTGTGAGAAGAGCAAGCGTCTTCGAACTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAGGAAGCAAAAGGCAGATGC
GGTGGCATTAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACGTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.232

100

0.672