Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   HMPREF0462_RS00100 Genome accession   NC_017375
Coordinates   14232..16595 (+) Length   787 a.a.
NCBI ID   WP_000888902.1    Uniprot ID   F4D2M1
Organism   Helicobacter pylori 83     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9232..21595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMPREF0462_RS00070 (HMPREF0462_0012) groES 9266..9622 (-) 357 WP_000671964.1 co-chaperone GroES -
  HMPREF0462_RS00075 (HMPREF0462_0013) dnaG 9897..11576 (+) 1680 WP_000601571.1 DNA primase -
  HMPREF0462_RS00080 (HMPREF0462_0014) - 11573..12625 (+) 1053 WP_000721161.1 MnmA/TRMU family protein -
  HMPREF0462_RS00085 (HMPREF0462_0015) - 12714..13541 (+) 828 WP_001154885.1 DUF5718 family protein -
  HMPREF0462_RS00090 (HMPREF0462_0016) comB2 13686..13967 (+) 282 WP_001272686.1 TrbC/VirB2 family protein Machinery gene
  HMPREF0462_RS00095 (HMPREF0462_0017) comB3 13967..14230 (+) 264 WP_000584955.1 hypothetical protein Machinery gene
  HMPREF0462_RS00100 (HMPREF0462_0018) comB4 14232..16595 (+) 2364 WP_000888902.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  HMPREF0462_RS00105 (HMPREF0462_0019) - 16897..18237 (+) 1341 WP_041201448.1 COG3014 family protein -
  HMPREF0462_RS00110 (HMPREF0462_0020) cheV1 18338..19303 (+) 966 WP_000785495.1 chemotaxis protein CheV1 -
  HMPREF0462_RS00115 (HMPREF0462_0021) nspC 19300..20517 (+) 1218 WP_000760877.1 carboxynorspermidine decarboxylase -
  HMPREF0462_RS00120 (HMPREF0462_0022) lpxE 20527..21099 (-) 573 WP_000819577.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90158.78 Da        Isoelectric Point: 8.7179

>NTDB_id=46994 HMPREF0462_RS00100 WP_000888902.1 14232..16595(+) (comB4) [Helicobacter pylori 83]
MLEELLSAIKQKVSNYFLGVLPKSYSMSEENNILGLYDENFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDHQQNIQSDSKYLQAILNQFENKEVYENQYFLVLESTHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLSSKEVLNFYVEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDAQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGKSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAILGAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFSSNAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNAKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHHFNVPLSETEIS
FLENTPLYARQVLVKNLGNGSSNMIDVSLEGLGRYLKIFNSDSSHVNKVKALQKDYPKEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=46994 HMPREF0462_RS00100 WP_000888902.1 14232..16595(+) (comB4) [Helicobacter pylori 83]
ATGTTAGAAGAGCTTTTGAGTGCTATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGAATTTCTTGCTCACTAAAAACGAAAACTTAGTGGGAATCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAGCAATTGCAAGATCTCTTCACCGAGCGCCAAATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGCGCGCCTTGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAACATTCAATCTGACTCTAA
ATACTTGCAAGCGATTTTGAATCAATTTGAAAATAAAGAAGTGTATGAAAATCAGTATTTTTTAGTTTTAGAAAGCACGC
ACTCTTTACAGGGCGTTTTAGAGCATAAGAAAAAATCTCTTATGCATGCCAATAGGGAAAATTTTAAGGATATTCTCTCT
TATAAAGCGCATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTACGCCCCCAAACTCTTAAGCTC
TAAAGAGGTTTTGAATTTTTATGTGGAATACATTAACGGGTTTGATCTCCCTTTAAAACCCCTAGTAGGGGGGTATTTGA
GCGACAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAAACGCC
CTTAGATATTATTTTTTCTATAGAGCCTATGAGCGTCAATAAAACGCTGAGTTTTTTAAAAGAGAGGGCCAAATTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTGCTAGAATACCAAGAACTGGTCAAAACCAAACGCCTATCCATGCAAAAATTCGCC
CTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGATGCTCAAACAAGCTTAGTTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAGGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACTTCTAAAGCCCTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACCTTAATTATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCATTTGACAGGATGCAGGGGCTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCAAATCTTTTAGCATCAATCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTTGATCTTGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGATTTTGGGTGCGATTAAGAGTCTTTATGAAACCTTATACCCTAAAGCTTTTAGTTTGTTAGACTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAATTGGGCTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCGTTCAGCTCCAACGCTTTTTTAAATGTGATAAACCTAGATGCCATCACCCAAAACCCTAAAGACTTAG
GGCTTTTAGCCTATTATTTGTTTTATAAAATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTATTGAACGCTAAAATTAACGCTTTAATCACGCAAGCCAGAAAAGCTAATGG
CGTGGTGGTGTTGGCTTTGCAAGACATTTACCAATTAAGCGGGGTTAAAAACGCTCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTACCCGCAAAAAAACGCTAGGGAGTTGAAACACCACTTCAATGTGCCTTTGAGCGAAACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAATGGGAGTTCTAACATGATTGATGT
GAGTTTGGAGGGTTTGGGGCGTTATTTGAAAATCTTTAATTCAGACTCTAGCCATGTGAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTAAAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F4D2M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.586

100

0.976

Multiple sequence alignment