Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JSQ86_RS04495 Genome accession   NZ_CP069707
Coordinates   909412..910392 (+) Length   326 a.a.
NCBI ID   WP_113078269.1    Uniprot ID   -
Organism   Escherichia coli strain E41     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 904412..915392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ86_RS04465 (JSQ86_04465) yggM 904927..905934 (+) 1008 WP_000745217.1 DUF1202 family protein -
  JSQ86_RS04470 (JSQ86_04470) hemW 906089..907225 (-) 1137 WP_097503509.1 radical SAM family heme chaperone HemW -
  JSQ86_RS04475 (JSQ86_04475) rdgB 907218..907811 (-) 594 WP_001174777.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JSQ86_RS04480 (JSQ86_04480) yggU 907819..908109 (-) 291 WP_000994920.1 DUF167 family protein YggU -
  JSQ86_RS04485 (JSQ86_04485) yggT 908106..908672 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JSQ86_RS04490 (JSQ86_04490) yggS 908690..909394 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JSQ86_RS04495 (JSQ86_04495) pilT 909412..910392 (+) 981 WP_113078269.1 type IV pilus twitching motility protein PilT Machinery gene
  JSQ86_RS04500 (JSQ86_04500) ruvX 910576..910992 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  JSQ86_RS04505 (JSQ86_04505) yqgE 910992..911555 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  JSQ86_RS04510 (JSQ86_04510) gshB 911664..912614 (-) 951 WP_000593273.1 glutathione synthase -
  JSQ86_RS04515 (JSQ86_04515) rsmE 912627..913358 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JSQ86_RS04520 (JSQ86_04520) endA 913438..914145 (-) 708 WP_000286500.1 deoxyribonuclease I -
  JSQ86_RS04525 (JSQ86_04525) yggI 914240..914737 (-) 498 WP_000858394.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35911.08 Da        Isoelectric Point: 5.9168

>NTDB_id=469834 JSQ86_RS04495 WP_113078269.1 909412..910392(+) (pilT) [Escherichia coli strain E41]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILALEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTCGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=469834 JSQ86_RS04495 WP_113078269.1 909412..910392(+) (pilT) [Escherichia coli strain E41]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGGCGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGTGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilU Acinetobacter baylyi ADP1

36.061

100

0.365