Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H1D00_RS09070 Genome accession   NZ_CP059330
Coordinates   1836552..1837106 (-) Length   184 a.a.
NCBI ID   WP_240021154.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0722     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831552..1842106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D00_RS09055 (H1D00_09110) sppA 1831630..1833486 (-) 1857 WP_240021152.1 signal peptide peptidase SppA -
  H1D00_RS09060 (H1D00_09115) - 1833594..1834157 (+) 564 WP_142777988.1 nitroreductase family protein -
  H1D00_RS09065 (H1D00_09120) - 1834228..1836552 (-) 2325 WP_240021153.1 Tex family protein -
  H1D00_RS09070 (H1D00_09125) pilF 1836552..1837106 (-) 555 WP_240021154.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H1D00_RS09075 (H1D00_09130) - 1837326..1837526 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H1D00_RS09080 (H1D00_09135) - 1837560..1837907 (+) 348 WP_006251771.1 helix-turn-helix transcriptional regulator -
  H1D00_RS09085 (H1D00_09140) - 1837948..1839108 (-) 1161 WP_240018540.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  H1D00_RS09090 (H1D00_09145) - 1839368..1840264 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  H1D00_RS09095 (H1D00_09150) - 1840277..1841020 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  H1D00_RS09100 (H1D00_09155) - 1841077..1841885 (-) 809 Protein_1789 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20967.61 Da        Isoelectric Point: 6.4644

>NTDB_id=468751 H1D00_RS09070 WP_240021154.1 1836552..1837106(-) (pilF) [Mannheimia haemolytica strain 0722]
MFAKFFPXMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=468751 H1D00_RS09070 WP_240021154.1 1836552..1837106(-) (pilF) [Mannheimia haemolytica strain 0722]
ATGTTTGCAAAATTTTTTCCANAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

48.235

92.391

0.446