Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H1D01_RS04380 Genome accession   NZ_CP059329
Coordinates   850737..851294 (-) Length   185 a.a.
NCBI ID   WP_240026066.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0727     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845737..856294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D01_RS04365 (H1D01_04410) sppA 845819..847675 (-) 1857 WP_240026063.1 signal peptide peptidase SppA -
  H1D01_RS04370 (H1D01_04415) - 847777..848340 (+) 564 WP_006249720.1 nitroreductase family protein -
  H1D01_RS04375 (H1D01_04420) - 848410..850737 (-) 2328 WP_240026064.1 Tex family protein -
  H1D01_RS04380 (H1D01_04425) pilF 850737..851294 (-) 558 WP_240026066.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H1D01_RS04385 (H1D01_04430) - 851513..851713 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H1D01_RS04390 (H1D01_04435) - 851747..852094 (+) 348 WP_006251771.1 helix-turn-helix transcriptional regulator -
  H1D01_RS04395 (H1D01_04440) - 852135..853295 (-) 1161 WP_240026067.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  H1D01_RS04400 (H1D01_04445) - 853555..854451 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  H1D01_RS04405 (H1D01_04450) - 854464..855207 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  H1D01_RS04410 (H1D01_04455) - 855263..856071 (-) 809 Protein_881 metal ABC transporter permease -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21098.98 Da        Isoelectric Point: 5.8119

>NTDB_id=468719 H1D01_RS04380 WP_240026066.1 850737..851294(-) (pilF) [Mannheimia haemolytica strain 0727]
MFAKFFPKMTACILIFLLAACSSLPXSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEXKDYLPYSVL
AYYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKXEPN
LQXIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 558 bp        

>NTDB_id=468719 H1D01_RS04380 WP_240026066.1 850737..851294(-) (pilF) [Mannheimia haemolytica strain 0727]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCNCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAANNAAAGATTACCTCCCTTATTCGGTGCTT
GCCTATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCG
CCCTGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGG
CTGTCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAANAAGAGCCGAAT
CTGCAANAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.766

100

0.638
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.674

92.973

0.443