Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H1D07_RS08115 Genome accession   NZ_CP059323
Coordinates   1677544..1678098 (-) Length   184 a.a.
NCBI ID   WP_240018538.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0768     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1672544..1683098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D07_RS08100 (H1D07_08145) sppA 1672622..1674478 (-) 1857 WP_240026063.1 signal peptide peptidase SppA -
  H1D07_RS08105 (H1D07_08150) - 1674586..1675149 (+) 564 WP_240026708.1 nitroreductase family protein -
  H1D07_RS08110 (H1D07_08155) - 1675220..1677544 (-) 2325 WP_240018537.1 Tex family protein -
  H1D07_RS08115 (H1D07_08160) pilF 1677544..1678098 (-) 555 WP_240018538.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H1D07_RS08120 (H1D07_08165) - 1678317..1678517 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  H1D07_RS08125 (H1D07_08170) - 1678551..1678898 (+) 348 WP_006251771.1 helix-turn-helix transcriptional regulator -
  H1D07_RS08130 (H1D07_08175) - 1678939..1680099 (-) 1161 WP_240018540.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  H1D07_RS08135 (H1D07_08180) - 1680359..1681255 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  H1D07_RS08140 (H1D07_08185) - 1681268..1682011 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  H1D07_RS08145 (H1D07_08190) - 1682069..1682877 (-) 809 Protein_1605 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20930.04 Da        Isoelectric Point: 5.8119

>NTDB_id=468590 H1D07_RS08115 WP_240018538.1 1677544..1678098(-) (pilF) [Mannheimia haemolytica strain 0768]
MFAKFFPKMTACILIXLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKXEPNL
QXIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=468590 H1D07_RS08115 WP_240018538.1 1677544..1678098(-) (pilF) [Mannheimia haemolytica strain 0768]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTNTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAANAAGAGCCGAATCTG
CAANAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.102

100

0.641
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.368

92.935

0.44