NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	HZT45_RS12110	Genome accession	NZ_CP059318
Coordinates	2530244..2530858 (-)	Length	204 a.a.
NCBI ID	WP_020956097.1	Uniprot ID	-
Organism	Bacillus velezensis strain BIOMA BV10
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2525244..2535858

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZT45_RS12080 (HZT45_12080)	rpsT	2525547..2525813 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HZT45_RS12085 (HZT45_12085)	holA	2525830..2526871 (-)	1042	Protein_2358	DNA polymerase III subunit delta	-
HZT45_RS12090 (HZT45_12090)	-	2526911..2527062 (-)	152	Protein_2359	hypothetical protein	-
HZT45_RS12095 (HZT45_12095)	-	2527103..2527237 (+)	135	WP_003152870.1	YqzM family protein	-
HZT45_RS12100 (HZT45_12100)	comEC	2527256..2529607 (-)	2352	WP_181467474.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HZT45_RS12105 (HZT45_12105)	-	2529608..2530177 (-)	570	WP_003152868.1	ComE operon protein 2	-
HZT45_RS12110 (HZT45_12110)	comEA	2530244..2530858 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
HZT45_RS12115 (HZT45_12115)	comER	2530917..2531738 (+)	822	WP_015240246.1	late competence protein ComER	-
HZT45_RS12120 (HZT45_12120)	-	2531807..2532544 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
HZT45_RS12125 (HZT45_12125)	rsfS	2532541..2532897 (-)	357	WP_007408260.1	ribosome silencing factor	-
HZT45_RS12130 (HZT45_12130)	yqeK	2532915..2533475 (-)	561	WP_181467475.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HZT45_RS12135 (HZT45_12135)	-	2533465..2534034 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
HZT45_RS12140 (HZT45_12140)	yhbY	2534045..2534335 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HZT45_RS12145 (HZT45_12145)	aroE	2534329..2535171 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22251.45 Da Isoelectric Point: 6.4940

                                >NTDB_id=468535 HZT45_RS12110 WP_020956097.1 2530244..2530858(-) (comEA) [Bacillus velezensis strain BIOMA BV10]

MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=468535 HZT45_RS12110 WP_020956097.1 2530244..2530858(-) (comEA) [Bacillus velezensis strain BIOMA BV10]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT

TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363

Detailed information NTG0468535