Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS074_RS07220 Genome accession   NZ_CP059245
Coordinates   1425894..1426448 (+) Length   184 a.a.
NCBI ID   WP_409038187.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0740     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1420894..1431448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS074_RS07190 (HS074_07135) - 1421112..1421921 (+) 810 Protein_1364 metal ABC transporter permease -
  HS074_RS07195 (HS074_07140) - 1421978..1422721 (-) 744 WP_240020776.1 metal ABC transporter ATP-binding protein -
  HS074_RS07200 (HS074_07145) - 1422734..1423630 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS074_RS07205 (HS074_07150) - 1423890..1425053 (+) 1164 WP_409038186.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS074_RS07210 (HS074_07155) - 1425094..1425441 (-) 348 WP_409037585.1 helix-turn-helix domain-containing protein -
  HS074_RS07215 - 1425520..1425675 (-) 156 Protein_1369 type II toxin-antitoxin system RelE family toxin -
  HS074_RS07220 (HS074_07165) pilF 1425894..1426448 (+) 555 WP_409038187.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS074_RS07225 (HS074_07170) - 1426448..1428772 (+) 2325 WP_006249721.1 Tex family protein -
  HS074_RS07230 (HS074_07175) - 1428844..1429407 (-) 564 WP_006249720.1 nitroreductase family protein -
  HS074_RS07235 (HS074_07180) sppA 1429510..1431366 (+) 1857 WP_006249718.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20935.81 Da        Isoelectric Point: 6.4644

>NTDB_id=468148 HS074_RS07220 WP_409038187.1 1425894..1426448(+) (pilF) [Mannheimia haemolytica strain 0740]
MFAKXFPKMTACILXFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAXKEPNL
XKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=468148 HS074_RS07220 WP_409038187.1 1425894..1426448(+) (pilF) [Mannheimia haemolytica strain 0740]
ATGTTTGCAAAATTNTTTCCAAAAATGACCGCTTGTATTCTGATNTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAANAAAGAGCCGAATCTG
CANAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(40-67)

Tetratricopeptide repeat (TPR_17)

(95-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.102

100

0.641
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446