Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS057_RS08540 Genome accession   NZ_CP059241
Coordinates   1669280..1669834 (+) Length   184 a.a.
NCBI ID   WP_409040125.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1664280..1674834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS057_RS08510 (HS057_08455) - 1664496..1665305 (+) 810 Protein_1625 metal ABC transporter permease -
  HS057_RS08515 (HS057_08460) - 1665362..1666108 (-) 747 WP_409040124.1 metal ABC transporter ATP-binding protein -
  HS057_RS08520 (HS057_08465) - 1666121..1667017 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS057_RS08525 (HS057_08470) - 1667277..1668437 (+) 1161 Protein_1628 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS057_RS08530 (HS057_08475) - 1668478..1668825 (-) 348 WP_409037585.1 helix-turn-helix domain-containing protein -
  HS057_RS08535 (HS057_08480) - 1668809..1669060 (-) 252 WP_006251772.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS057_RS08540 (HS057_08485) pilF 1669280..1669834 (+) 555 WP_409040125.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS057_RS08545 (HS057_08490) - 1669834..1672158 (+) 2325 WP_240020784.1 Tex family protein -
  HS057_RS08550 (HS057_08495) - 1672230..1672793 (-) 564 WP_006249720.1 nitroreductase family protein -
  HS057_RS08555 (HS057_08500) sppA 1672896..1674755 (+) 1860 WP_409038867.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20958.37 Da        Isoelectric Point: 6.4644

>NTDB_id=468058 HS057_RS08540 WP_409040125.1 1669280..1669834(+) (pilF) [Mannheimia haemolytica strain 0019]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAXKEPNL
XKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=468058 HS057_RS08540 WP_409040125.1 1669280..1669834(+) (pilF) [Mannheimia haemolytica strain 0019]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAANAAAGAGCCGAATCTG
CANAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446