Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS058_RS04580 Genome accession   NZ_CP059240
Coordinates   880333..880887 (-) Length   184 a.a.
NCBI ID   WP_409044883.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875333..885887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS058_RS04565 (HS058_04530) sppA 875417..877273 (-) 1857 WP_240021152.1 signal peptide peptidase SppA -
  HS058_RS04570 (HS058_04535) - 877376..877939 (+) 564 WP_006249720.1 nitroreductase family protein -
  HS058_RS04575 (HS058_04540) - 878009..880333 (-) 2325 WP_006249721.1 Tex family protein -
  HS058_RS04580 (HS058_04545) pilF 880333..880887 (-) 555 WP_409044883.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS058_RS04585 (HS058_04550) - 881107..881307 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS058_RS04590 (HS058_04555) - 881341..881688 (+) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  HS058_RS04595 (HS058_04560) - 881729..882889 (-) 1161 WP_409044884.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS058_RS04600 (HS058_04565) - 883149..884045 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS058_RS04605 (HS058_04570) - 884058..884801 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  HS058_RS04610 (HS058_04575) - 884859..885668 (-) 810 Protein_914 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20967.61 Da        Isoelectric Point: 6.4644

>NTDB_id=468028 HS058_RS04580 WP_409044883.1 880333..880887(-) (pilF) [Mannheimia haemolytica strain 0039]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKXEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=468028 HS058_RS04580 WP_409044883.1 880333..880887(-) (pilF) [Mannheimia haemolytica strain 0039]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAANAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446