Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS060_RS08855 Genome accession   NZ_CP059238
Coordinates   1812794..1813351 (-) Length   185 a.a.
NCBI ID   WP_240026066.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0152     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1807794..1818351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS060_RS08840 (HS060_08825) sppA 1807869..1809725 (-) 1857 WP_240021152.1 signal peptide peptidase SppA -
  HS060_RS08845 (HS060_08830) - 1809833..1810396 (+) 564 WP_006249720.1 nitroreductase family protein -
  HS060_RS08850 (HS060_08835) - 1810467..1812794 (-) 2328 WP_409035921.1 Tex family protein -
  HS060_RS08855 (HS060_08840) pilF 1812794..1813351 (-) 558 WP_240026066.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS060_RS08860 (HS060_08845) - 1813571..1813771 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS060_RS08865 (HS060_08850) - 1813805..1814152 (+) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  HS060_RS08870 (HS060_08855) - 1814193..1815353 (-) 1161 WP_240018540.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS060_RS08875 (HS060_08860) - 1815613..1816509 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS060_RS08880 (HS060_08865) - 1816522..1817265 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  HS060_RS08885 (HS060_08870) - 1817322..1818129 (-) 808 Protein_1731 metal ABC transporter permease -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21098.98 Da        Isoelectric Point: 5.8119

>NTDB_id=467992 HS060_RS08855 WP_240026066.1 1812794..1813351(-) (pilF) [Mannheimia haemolytica strain 0152]
MFAKFFPKMTACILIFLLAACSSLPXSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEXKDYLPYSVL
AYYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKXEPN
LQXIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 558 bp        

>NTDB_id=467992 HS060_RS08855 WP_240026066.1 1812794..1813351(-) (pilF) [Mannheimia haemolytica strain 0152]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCNCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAANNAAAGATTACCTCCCTTATTCGGTGCTT
GCCTATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCG
CCCTGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGG
CTGTCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAANAAGAGCCGAAT
CTGCAANAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.766

100

0.638
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.674

92.973

0.443