Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS065_RS01350 Genome accession   NZ_CP059233
Coordinates   285557..286117 (+) Length   186 a.a.
NCBI ID   WP_409045831.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0627     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 280557..291117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS065_RS01320 (HS065_01315) - 280775..281583 (+) 809 Protein_254 metal ABC transporter permease -
  HS065_RS01325 (HS065_01320) - 281642..282385 (-) 744 WP_240020776.1 metal ABC transporter ATP-binding protein -
  HS065_RS01330 (HS065_01325) - 282398..283294 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS065_RS01335 (HS065_01330) - 283554..284714 (+) 1161 WP_409041831.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS065_RS01340 (HS065_01335) - 284755..285102 (-) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  HS065_RS01345 (HS065_01340) - 285086..285337 (-) 252 WP_006251772.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS065_RS01350 (HS065_01345) pilF 285557..286117 (+) 561 WP_409045831.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS065_RS01355 (HS065_01350) - 286117..288441 (+) 2325 WP_409038866.1 Tex family protein -
  HS065_RS01360 (HS065_01355) - 288512..289075 (-) 564 WP_006249720.1 nitroreductase family protein -
  HS065_RS01365 (HS065_01360) sppA 289178..291037 (+) 1860 WP_409045832.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21196.14 Da        Isoelectric Point: 6.4644

>NTDB_id=467866 HS065_RS01350 WP_409045831.1 285557..286117(+) (pilF) [Mannheimia haemolytica strain 0627]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLAXLAYLEQNDFPKAKQNIDKALAHDEXKDYLPYSV
LAYYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAXKEP
NLXKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 561 bp        

>NTDB_id=467866 HS065_RS01350 WP_409045831.1 285557..286117(+) (pilF) [Mannheimia haemolytica strain 0627]
ATGTTTGCAAAATTTTTTCCNAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTNNTTTAGCTTATCTGGAGCAAA
ATGATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAANNAAAAGATTACCTCCCTTATTCGGTG
CTTGCCTATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAA
CCGCCCTGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGC
AGGCTGTCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAANAAAGAGCCG
AATCTGCANAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

62.963

100

0.64
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.399

93.011

0.441