Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS071_RS03275 Genome accession   NZ_CP059228
Coordinates   654547..655104 (+) Length   185 a.a.
NCBI ID   WP_240020782.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0715     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 649547..660104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS071_RS03245 (HS071_03245) - 649766..650576 (+) 811 Protein_619 metal ABC transporter permease -
  HS071_RS03250 (HS071_03250) - 650633..651376 (-) 744 WP_240020776.1 metal ABC transporter ATP-binding protein -
  HS071_RS03255 (HS071_03255) - 651389..652285 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS071_RS03260 (HS071_03260) - 652545..653705 (+) 1161 WP_409041831.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS071_RS03265 (HS071_03265) - 653746..654093 (-) 348 WP_409037585.1 helix-turn-helix domain-containing protein -
  HS071_RS03270 (HS071_03270) - 654127..654327 (-) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS071_RS03275 (HS071_03275) pilF 654547..655104 (+) 558 WP_240020782.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS071_RS03280 (HS071_03280) - 655104..657428 (+) 2325 WP_006249721.1 Tex family protein -
  HS071_RS03285 (HS071_03285) - 657499..658062 (-) 564 WP_240030279.1 nitroreductase family protein -
  HS071_RS03290 (HS071_03290) sppA 658165..660021 (+) 1857 WP_006249718.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21077.26 Da        Isoelectric Point: 6.4644

>NTDB_id=467823 HS071_RS03275 WP_240020782.1 654547..655104(+) (pilF) [Mannheimia haemolytica strain 0715]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEXKDYLPYSVL
AYYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAXKEPN
LXKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 558 bp        

>NTDB_id=467823 HS071_RS03275 WP_240020782.1 654547..655104(+) (pilF) [Mannheimia haemolytica strain 0715]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAANNAAAAGATTACCTCCCTTATTCGGTGCTT
GCCTATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCG
CCCTGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGG
CTGTCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAANAAAGAGCCGAAT
CTGCANAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.298

100

0.643
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.674

92.973

0.443