Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS072_RS02785 Genome accession   NZ_CP059227
Coordinates   558748..559305 (-) Length   185 a.a.
NCBI ID   WP_409044312.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0731     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 553748..564305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS072_RS02770 (HS072_02785) sppA 553831..555687 (-) 1857 WP_240026063.1 signal peptide peptidase SppA -
  HS072_RS02775 (HS072_02790) - 555790..556353 (+) 564 WP_006249720.1 nitroreductase family protein -
  HS072_RS02780 (HS072_02795) - 556424..558748 (-) 2325 WP_409043036.1 Tex family protein -
  HS072_RS02785 (HS072_02800) pilF 558748..559305 (-) 558 WP_409044312.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS072_RS02790 (HS072_02805) - 559524..559724 (+) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS072_RS02795 (HS072_02810) - 559758..560105 (+) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  HS072_RS02800 (HS072_02815) - 560146..561309 (-) 1164 WP_409044313.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS072_RS02805 (HS072_02820) - 561569..562465 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS072_RS02810 (HS072_02825) - 562478..563221 (+) 744 WP_240018541.1 metal ABC transporter ATP-binding protein -
  HS072_RS02815 (HS072_02830) - 563279..564087 (-) 809 Protein_560 metal ABC transporter permease -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21108.27 Da        Isoelectric Point: 6.4644

>NTDB_id=467799 HS072_RS02785 WP_409044312.1 558748..559305(-) (pilF) [Mannheimia haemolytica strain 0731]
MFAKFFPKMTACILIFLLAACSSLPXSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEXKDYLPYSVL
AYYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKXEPN
LQKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 558 bp        

>NTDB_id=467799 HS072_RS02785 WP_409044312.1 558748..559305(-) (pilF) [Mannheimia haemolytica strain 0731]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCNCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAANNAAAGATTACCTCCCTTATTCGGTGCTT
GCCTATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCG
CCCTGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGG
CTGTCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAANAAGAGCCGAAT
CTGCAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(96-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.298

100

0.643
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.674

92.973

0.443