Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HS073_RS05970 Genome accession   NZ_CP059226
Coordinates   1180143..1180697 (+) Length   184 a.a.
NCBI ID   WP_409037273.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0739     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1175143..1185697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS073_RS05940 (HS073_05910) - 1175362..1176170 (+) 809 Protein_1123 metal ABC transporter permease -
  HS073_RS05945 (HS073_05915) - 1176229..1176972 (-) 744 WP_240020776.1 metal ABC transporter ATP-binding protein -
  HS073_RS05950 (HS073_05920) - 1176985..1177881 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  HS073_RS05955 (HS073_05925) - 1178141..1179301 (+) 1161 Protein_1126 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HS073_RS05960 (HS073_05930) - 1179342..1179689 (-) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  HS073_RS05965 (HS073_05935) - 1179723..1179923 (-) 201 WP_240018539.1 type II toxin-antitoxin system RelE/ParE family toxin -
  HS073_RS05970 (HS073_05940) pilF 1180143..1180697 (+) 555 WP_409037273.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HS073_RS05975 (HS073_05945) - 1180697..1183024 (+) 2328 WP_409037274.1 Tex family protein -
  HS073_RS05980 (HS073_05950) - 1183095..1183658 (-) 564 WP_006249720.1 nitroreductase family protein -
  HS073_RS05985 (HS073_05955) sppA 1183761..1185620 (+) 1860 WP_409037275.1 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20967.66 Da        Isoelectric Point: 7.0805

>NTDB_id=467782 HS073_RS05970 WP_409037273.1 1180143..1180697(+) (pilF) [Mannheimia haemolytica strain 0739]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
XKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=467782 HS073_RS05970 WP_409037273.1 1180143..1180697(+) (pilF) [Mannheimia haemolytica strain 0739]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CANAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446