Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H0242_RS21715 Genome accession   NZ_CP059148
Coordinates   4248498..4249541 (-) Length   347 a.a.
NCBI ID   WP_001013181.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4243498..4254541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0242_RS21670 (H0242_21415) - 4243931..4244131 (-) 201 WP_000106083.1 YqzE family protein -
  H0242_RS21675 (H0242_21420) aroK 4244170..4244667 (-) 498 WP_000836655.1 shikimate kinase AroK -
  H0242_RS21680 (H0242_21425) - 4244783..4245433 (-) 651 WP_000183838.1 2OG-Fe(II) oxygenase -
  H0242_RS21685 (H0242_21430) comGG 4245608..4245979 (-) 372 WP_001214887.1 competence type IV pilus minor pilin ComGG -
  H0242_RS21690 (H0242_21435) comGF 4245976..4246446 (-) 471 WP_000923095.1 competence type IV pilus minor pilin ComGF -
  H0242_RS21695 (H0242_21440) - 4246416..4246718 (-) 303 WP_000829466.1 hypothetical protein -
  H0242_RS21700 (H0242_21445) comGD 4246711..4247166 (-) 456 WP_042972675.1 competence type IV pilus minor pilin ComGD -
  H0242_RS21705 (H0242_21450) comGC 4247163..4247462 (-) 300 WP_001178681.1 competence type IV pilus major pilin ComGC -
  H0242_RS21710 (H0242_21455) comGB 4247474..4248511 (-) 1038 WP_042973412.1 competence type IV pilus assembly protein ComGB -
  H0242_RS21715 (H0242_21460) comGA 4248498..4249541 (-) 1044 WP_001013181.1 competence type IV pilus ATPase ComGA Machinery gene
  H0242_RS21720 (H0242_21465) - 4249747..4250442 (+) 696 WP_000434108.1 helix-turn-helix transcriptional regulator -
  H0242_RS21725 (H0242_21470) - 4250569..4250811 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  H0242_RS21730 (H0242_21475) - 4250919..4252313 (+) 1395 WP_001094344.1 L-cystine transporter -
  H0242_RS21735 - 4252392..4252784 (-) 393 Protein_4230 hypothetical protein -
  H0242_RS21740 (H0242_21485) - 4252872..4253087 (-) 216 WP_001008333.1 DUF3912 family protein -
  H0242_RS21745 (H0242_21490) - 4253340..4253651 (+) 312 WP_001093240.1 hypothetical protein -
  H0242_RS21750 (H0242_21495) - 4253686..4254174 (-) 489 WP_000764268.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39168.55 Da        Isoelectric Point: 8.7212

>NTDB_id=467379 H0242_RS21715 WP_001013181.1 4248498..4249541(-) (comGA) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=467379 H0242_RS21715 WP_001013181.1 4248498..4249541(-) (comGA) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTAACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369