Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H0242_RS00535 Genome accession   NZ_CP059148
Coordinates   99136..100512 (+) Length   458 a.a.
NCBI ID   WP_001085199.1    Uniprot ID   A0A9W5QZX4
Organism   Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94136..105512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0242_RS00515 (H0242_00515) ctsR 94333..94794 (+) 462 WP_001244560.1 transcriptional regulator CtsR -
  H0242_RS00520 (H0242_00520) - 94965..95513 (+) 549 WP_000128386.1 UvrB/UvrC motif-containing protein -
  H0242_RS00525 (H0242_00525) - 95518..96582 (+) 1065 WP_000050834.1 protein arginine kinase -
  H0242_RS00530 (H0242_00530) clpC 96605..99040 (+) 2436 WP_000971173.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  H0242_RS00535 (H0242_00535) radA 99136..100512 (+) 1377 WP_001085199.1 DNA repair protein RadA Machinery gene
  H0242_RS00540 (H0242_00540) disA 100516..101589 (+) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  H0242_RS00545 (H0242_00545) - 101751..102854 (+) 1104 WP_000919681.1 PIN/TRAM domain-containing protein -
  H0242_RS00550 (H0242_00550) ispD 102871..103551 (+) 681 WP_000288311.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  H0242_RS00555 (H0242_00555) ispF 103668..104144 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49899.60 Da        Isoelectric Point: 6.9053

>NTDB_id=467350 H0242_RS00535 WP_001085199.1 99136..100512(+) (radA) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=467350 H0242_RS00535 WP_001085199.1 99136..100512(+) (radA) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGTAAATGTCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAGCCAGTTGTATCATCCAGGCGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACCGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AATCGTGTGCTTGGTGGCGGAATTGTAGATGGGTCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
ACTATTACAAATTTCATCACAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACCTGCAGCGTATTGCA
GCACATATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACAATACATTTACCTGAGGTGACGTCAGC
ACCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TCGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACGTATCGTATTTTACGAGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCCGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GATCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCTGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
TTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGGGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633