Detailed information NTG0467307

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: H0I83_RS05790
• Genome accession: NZ_CP059141
• Coordinates: 1123441..1127166 (+)
• Length: 1241 a.a.
• NCBI ID: WP_086790538.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis serovar fukuokaensis strain 4AP1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1118441..1132166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0I83_RS05775 (H0I83_05715)	-	1118600..1119193 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
H0I83_RS05780 (H0I83_05720)	lepB	1119250..1119813 (+)	564	WP_000751896.1	signal peptidase I	-
H0I83_RS05785 (H0I83_05725)	addB	1119929..1123444 (+)	3516	WP_086405973.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
H0I83_RS05790 (H0I83_05730)	addA	1123441..1127166 (+)	3726	WP_086790538.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
H0I83_RS05795 (H0I83_05735)	-	1127179..1127466 (+)	288	WP_000255722.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
H0I83_RS05800 (H0I83_05740)	gerPF	1127604..1127819 (-)	216	WP_001141566.1	spore germination protein GerPF	-
H0I83_RS05805 (H0I83_05745)	-	1127862..1128248 (-)	387	WP_000902320.1	spore germination protein GerPE	-
H0I83_RS05810 (H0I83_05750)	gerPD	1128264..1128458 (-)	195	WP_001052807.1	spore germination protein GerPD	-
H0I83_RS05815 (H0I83_05755)	gerPC	1128465..1129079 (-)	615	WP_001070761.1	spore germination protein GerPC	-
H0I83_RS05820 (H0I83_05760)	gerPB	1129147..1129353 (-)	207	WP_001012510.1	spore germination protein GerPB	-
H0I83_RS05825 (H0I83_05765)	gerPA	1129368..1129589 (-)	222	WP_001111188.1	spore germination protein GerPA	-
H0I83_RS05830 (H0I83_05770)	-	1129686..1129865 (-)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
H0I83_RS05835 (H0I83_05775)	-	1130112..1131011 (+)	900	WP_001213077.1	fumarylacetoacetate hydrolase family protein	-
H0I83_RS05840 (H0I83_05780)	-	1131047..1131331 (-)	285	WP_000926871.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142732.95 Da
• Isoelectric Point: 4.7735

>NTDB_id=467307 H0I83_RS05790 WP_086790538.1 1123441..1127166(+) (addA) [Bacillus thuringiensis serovar fukuokaensis strain 4AP1]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGELNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIEQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGLLYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVEGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSYRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=467307 H0I83_RS05790 WP_086790538.1 1123441..1127166(+) (addA) [Bacillus thuringiensis serovar fukuokaensis strain 4AP1]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCGCAAGAGATGAAAAATCGAATC
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAGCATGTAAGAAAGCAACTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTGGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAACTGAATCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTGTTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCAATTTATCGTTTCCGATTAGCAG
AACCAGGCCTGTTTCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGATTTGGCGAAA
AACTTCCGTAGTCGTCATGAAGTGCTAGCAGGTACGAACTTCATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGATGCTGACGCTGAATTAAAACTAGGCGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAATTACTATGTA
TTGAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCAATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTTGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCAACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCAATCGTTGGATTAAACGATGAAGACCTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTTGCTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAGGAACAAGAAC
TTCATGAAAAACTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGCGAGACAGGCTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGGTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGGGCTCTCGGTGAACAAGAAGACGTTGTTCGCATCATGACGATC
CATAAAAGTAAAGGGCTAGAGTTCCCAGTTGTATTTGTAGCTGGACTCGGCCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTCCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATACACGACATTAT
CACAGCTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACGAGCTGGAAA
GTAGAAGTTGTGGAAGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCAACATCTTATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCGCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GATTTAGGTAAAAGGGTACTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAGTG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534