Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   H0I83_RS05785 Genome accession   NZ_CP059141
Coordinates   1119929..1123444 (+) Length   1171 a.a.
NCBI ID   WP_086405973.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar fukuokaensis strain 4AP1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1114929..1128444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0I83_RS05750 (H0I83_05690) - 1115387..1115881 (-) 495 WP_086405971.1 DUF6584 family protein -
  H0I83_RS05755 (H0I83_05695) cspA 1116165..1116368 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  H0I83_RS05760 (H0I83_05700) - 1116569..1116772 (-) 204 WP_061666462.1 hypothetical protein -
  H0I83_RS05765 (H0I83_05705) - 1117123..1117308 (-) 186 WP_000391373.1 hypothetical protein -
  H0I83_RS05770 (H0I83_05710) - 1117592..1118173 (+) 582 WP_000444446.1 competence protein ComK -
  H0I83_RS05775 (H0I83_05715) - 1118600..1119193 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  H0I83_RS05780 (H0I83_05720) lepB 1119250..1119813 (+) 564 WP_000751896.1 signal peptidase I -
  H0I83_RS05785 (H0I83_05725) addB 1119929..1123444 (+) 3516 WP_086405973.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  H0I83_RS05790 (H0I83_05730) addA 1123441..1127166 (+) 3726 WP_086790538.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  H0I83_RS05795 (H0I83_05735) - 1127179..1127466 (+) 288 WP_000255722.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  H0I83_RS05800 (H0I83_05740) gerPF 1127604..1127819 (-) 216 WP_001141566.1 spore germination protein GerPF -
  H0I83_RS05805 (H0I83_05745) - 1127862..1128248 (-) 387 WP_000902320.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134060.37 Da        Isoelectric Point: 5.2131

>NTDB_id=467306 H0I83_RS05785 WP_086405973.1 1119929..1123444(+) (addB) [Bacillus thuringiensis serovar fukuokaensis strain 4AP1]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGRYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGAKVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTAEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEILGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSAAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=467306 H0I83_RS05785 WP_086405973.1 1119929..1123444(+) (addB) [Bacillus thuringiensis serovar fukuokaensis strain 4AP1]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGGGGGTCTATTCGGGCACAAGTTTTTAGCTTTTCACGATTAGCGTGGAAGGTACTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATCGCGGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGCTATTATACGATGATTTTGAACGTGCTTTAATTGGAAGATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATCGTAAGACAGCTTATGATTTGCGGAGCGAAAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTAGC
ACGTGAAGAGAAAATAGAAATTGAAAAAACAATCCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGATGAAAAATATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGTTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTGCTGAAGATCCATGGATGTATCGGCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTTTAGAAGAGCTGGATGTTCCGAAGAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGACTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAGATACTTGGTGAAGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TCATTCCAGCAGCACCTATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAATGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTGCAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGATCACTTCCGCCGATGG
AATTTTCATTACCAAATGGTGTAAAGATGGAAGTAGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAGAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCGGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494