Detailed information NTG0466108

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: HZ310_RS14140
• Genome accession: NZ_CP058951
• Coordinates: 2689271..2692969 (-)
• Length: 1232 a.a.
• NCBI ID: WP_180309271.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain UAMX
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2684271..2697969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZ310_RS14125 (HZ310_14125)	-	2684358..2684657 (-)	300	WP_180309268.1	HNH endonuclease	-
HZ310_RS14130 (HZ310_14130)	-	2684677..2688072 (-)	3396	WP_180309269.1	AAA family ATPase	-
HZ310_RS14135 (HZ310_14135)	sbcD	2688069..2689244 (-)	1176	WP_180309270.1	exonuclease subunit SbcD	-
HZ310_RS14140 (HZ310_14140)	addA	2689271..2692969 (-)	3699	WP_180309271.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
HZ310_RS14145 (HZ310_14145)	addB	2692947..2696456 (-)	3510	WP_180309272.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
HZ310_RS14150 (HZ310_14150)	-	2696621..2697067 (+)	447	WP_061407295.1	ferritin-like domain-containing protein	-
HZ310_RS14155 (HZ310_14155)	-	2697263..2697673 (-)	411	WP_180309273.1	DoxX family protein	-

Sequence

Protein

• Length: 1232 a.a.
• Molecular weight: 142212.20 Da
• Isoelectric Point: 5.0692

>NTDB_id=466108 HZ310_RS14140 WP_180309271.1 2689271..2692969(-) (addA) [Bacillus pumilus strain UAMX]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQILRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQQEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGVPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQFLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAASGEEWLLPDFERYQSKTYLDFIGPALIRHQAMASVLEEAGDVVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALMDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYRHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAVESILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3699 bp

>NTDB_id=466108 HZ310_RS14140 WP_180309271.1 2689271..2692969(-) (addA) [Bacillus pumilus strain UAMX]
ATGCAAATACCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGACCGTCTGCTTGTTGTAACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAACCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGATTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCCG
ATCAAACAGAAGGAGAACTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAACTGGTTGACCGTTACACGTCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCAAGATCGCATCCATCCCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGTCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAACTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTTTATTAGAAAAAGCCACAGATGCACGTAATCAAGCGAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAGCATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTTGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATTGAAACAGAAGCTGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTTGTTGATGAATATCAGGATACAAACCTTGTACAAGAAACCATTTTAAAGCTTGTATCTAAAGGAGA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCTATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTGAATAAAAACTTCCGC
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCAGGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAACTTACAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCGTGGGCTCCGCAAATGATGGAGGAATTAAAGAAGCAGGGAGTCCCCGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCTTTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTCTTGTTCATTTAGATGAAAATGAATTGGCTCTGATTCGAACAAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTTATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAGCTAG
AGCGATTTTTCCAATTCCTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGACACGCAGTATCTTGATTATGTTGGCGGGATGCCTGGAGGTAAGCAGCGGCAGGCGAATCTTCGTGCCCTTTATGA
CAGGGCCAAGCAATATGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATACACAGCAGTAAAGGG
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTATTTACTTGA
TAAAGAACTAGGGTTTGGAAGCAAGTACATCCATCCTGAACTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGAGTCCTATATGTGGCACTCACAAGGGCTAAAGAAAAATTA
TTTCTGGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCATCCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCAAAAACATACTTAGATTTCATTGGACCTGCCCTTATCCGTCATCAAGCGATGGCAT
CTGTTTTAGAAGAAGCAGGAGATGTTGTTCTTTCACATCCGTCTACATTCACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAGAAGGAGCAAGACGAGATTGTACAAGCATTGATGGATGGTCTGCCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGGCTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTTCCTTCAGAGGAGCCTTATGATGAATCACGGATAGGACACTTGCTCGAATCTTTACAGCAGC
GAGATTTGCTAACAGATGAGCAGATTCAGTCAATTAATCAAGAAGGAATTGCATCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTGAAACGAGAGGTGTCTTTTAGTATGGTGTTACCTGTTAAAGAGGTATACCGTCATAT
AGATACTGAGGGAGAGCCTGTTCTGATTCAAGGAATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGTTGAATCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCATTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTCGATGGGAA
TTTAGAAATCTCTTTATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1224)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.1	100	0.663