Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   HZ310_RS14145 Genome accession   NZ_CP058951
Coordinates   2692947..2696456 (-) Length   1169 a.a.
NCBI ID   WP_180309272.1    Uniprot ID   -
Organism   Bacillus pumilus strain UAMX     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2687947..2701456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ310_RS14135 (HZ310_14135) sbcD 2688069..2689244 (-) 1176 WP_180309270.1 exonuclease subunit SbcD -
  HZ310_RS14140 (HZ310_14140) addA 2689271..2692969 (-) 3699 WP_180309271.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  HZ310_RS14145 (HZ310_14145) addB 2692947..2696456 (-) 3510 WP_180309272.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  HZ310_RS14150 (HZ310_14150) - 2696621..2697067 (+) 447 WP_061407295.1 ferritin-like domain-containing protein -
  HZ310_RS14155 (HZ310_14155) - 2697263..2697673 (-) 411 WP_180309273.1 DoxX family protein -
  HZ310_RS14160 (HZ310_14160) - 2697730..2698569 (-) 840 WP_058014364.1 M56 family metallopeptidase -
  HZ310_RS14165 (HZ310_14165) - 2698562..2698984 (-) 423 WP_058014365.1 BlaI/MecI/CopY family transcriptional regulator -
  HZ310_RS14170 (HZ310_14170) - 2699251..2700390 (+) 1140 WP_144462404.1 AbrB family transcriptional regulator -
  HZ310_RS14175 (HZ310_14175) lepB 2700426..2700938 (-) 513 WP_144462407.1 signal peptidase I -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134791.62 Da        Isoelectric Point: 5.3914

>NTDB_id=466109 HZ310_RS14145 WP_180309272.1 2692947..2696456(-) (addB) [Bacillus pumilus strain UAMX]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMPPEEIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLAQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECGADISETIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKRLKNAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAEMA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDNEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKQNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIYKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSKDHFHILTQHVRRTFEEAGERITNGEVTINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=466109 HZ310_RS14145 WP_180309272.1 2692947..2696456(-) (addB) [Bacillus pumilus strain UAMX]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCCGGTGGAATGATCAGAGCTAAAGTTTTTAGTTTTACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGT
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCCAGTGATAAGCCAGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCCGCCTGAGGAAATTGAGAAAATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTGCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACATATTTATATCGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTCCATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCGAA
AGAGTGCGGAGCTGACATTTCTGAAACCATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCAGTACAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGACATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTCCGAGATATCTCGAT
TGTAGCGAGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGACATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAATGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATCGAAATGGAACAAATGCTAAATGATGTAAAAGAATGG
ATCGCGCCCCCGCTTTACCAGCTGCAGAAAAGACTTAAAAATGCACAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCTGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCTTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGATCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAACGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGATGGCA
GAGACAGGAAAAGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTTTATTGCCTTCAACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTATTAGATCCAGAACAGCTGGAAGACAATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACTGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGGGATGTATCAAAAAACATCATGTCAAGTTTGTTCTTTACAAATCGAGCGAAGC
CTTTAAAGCAAAATGTCACAAAAGAGCTTTACGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGTTTAAAACTTAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAGTTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTAATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTTGAGTTAGGTTTCGGGGGGCAAGGACCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAACTTGTCGGAAGAATTGACAGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTACTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCAATTCCGCTTGCAGAAGATGAAATTGAGCAGGAAATATACAAGAAATTTAAAATGAAGGGGTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCTGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTAAAGATCATTTCCATATACTCACACAACACGTTCGGC
GTACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCACAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCTTTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGAGTGCTCACCTCTGA
AAAGGACGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.978

99.316

0.655