Detailed information NTG0464558

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JNO62_RS02995
• Genome accession: NZ_CP069245
• Coordinates: 648556..649437 (-)
• Length: 293 a.a.
• NCBI ID: WP_029240316.1
• Uniprot ID: A0ABX7ZX66
• Organism: Ralstonia pseudosolanacearum strain PeaFJ1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 643556..654437

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JNO62_RS02965 (JNO62_02955)	argJ	644266..645495 (+)	1230	WP_011002739.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
JNO62_RS02970 (JNO62_02960)	-	645511..646395 (+)	885	WP_011002738.1	ATP-binding protein	-
JNO62_RS02975 (JNO62_02965)	-	646373..646834 (+)	462	WP_011002737.1	NUDIX domain-containing protein	-
JNO62_RS02980 (JNO62_02970)	yacG	646827..647042 (-)	216	WP_011002736.1	DNA gyrase inhibitor YacG	-
JNO62_RS02985 (JNO62_02975)	zapD	647053..647811 (-)	759	WP_016723794.1	cell division protein ZapD	-
JNO62_RS02990 (JNO62_02980)	coaE	647929..648543 (-)	615	WP_011002734.1	dephospho-CoA kinase	-
JNO62_RS02995 (JNO62_02985)	pilD	648556..649437 (-)	882	WP_029240316.1	prepilin peptidase	Machinery gene
JNO62_RS03000 (JNO62_02990)	pilC	649440..650705 (-)	1266	WP_019718222.1	type II secretion system F family protein	Machinery gene
JNO62_RS03005 (JNO62_02995)	pilF	650750..652477 (-)	1728	WP_071013048.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JNO62_RS03010 (JNO62_03000)	-	652725..654002 (-)	1278	WP_016723792.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31573.54 Da
• Isoelectric Point: 8.3415

>NTDB_id=464558 JNO62_RS02995 WP_029240316.1 648556..649437(-) (pilD) [Ralstonia pseudosolanacearum strain PeaFJ1]
MPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCGH
QIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLSS
VVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=464558 JNO62_RS02995 WP_029240316.1 648556..649437(-) (pilD) [Ralstonia pseudosolanacearum strain PeaFJ1]
ATGCCCGGAATGTTCGTCATTCCCACGCTGGCGCAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGGCTT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCAACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTGCGCGGCCGCTGCAGCGCCTGCAAGACCCCCAT
CTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACCGGCGTACTGACCGGCGCCTGCCTGTGGCACTTCGGACCGACCTGGG
TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAGCTCCTGCCCGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCAGCATGTTCGCGCGGCTGCCGGACGCGGTAAT
CGGCGCGACGGCGGGGTATCTGTTCCTGTGGGCCATCTACTGGGCCTATAAGCTCCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGGCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGCCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTCTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTGATCGCGCTGCTGGCCGGCCCGCAACTGGTGGCGCTGACGGGGCTCGCGCCACTGCTGGCGCGGT
AA

Domains

Predicted by InterProScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.453	90.444	0.474
pilD	Vibrio campbellii strain DS40M4	48.736	94.539	0.461
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	45.865	90.785	0.416
pilD	Acinetobacter baumannii D1279779	45.865	90.785	0.416