Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   HWH77_RS16325 Genome accession   NZ_CP058216
Coordinates   3210492..3212321 (+) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus velezensis strain LF01     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 3205492..3217321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWH77_RS16295 (HWH77_16300) - 3206155..3206733 (+) 579 WP_003155035.1 GNAT family N-acetyltransferase -
  HWH77_RS16300 (HWH77_16305) spx 3206912..3207307 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  HWH77_RS16305 (HWH77_16310) - 3207365..3208021 (-) 657 WP_003155033.1 TerC family protein -
  HWH77_RS19950 (HWH77_16315) - 3208204..3208314 (+) 111 Protein_3179 adaptor protein MecA -
  HWH77_RS16315 (HWH77_16320) mecA 3208297..3208953 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  HWH77_RS16320 (HWH77_16325) - 3209104..3210264 (+) 1161 WP_161503321.1 competence protein CoiA family protein -
  HWH77_RS16325 (HWH77_16330) pepF 3210492..3212321 (+) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  HWH77_RS16330 (HWH77_16335) - 3212359..3212526 (-) 168 WP_003155026.1 hypothetical protein -
  HWH77_RS16335 (HWH77_16340) spxH 3212812..3213714 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  HWH77_RS16340 (HWH77_16345) - 3213711..3214109 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  HWH77_RS16345 (HWH77_16350) - 3214338..3215024 (-) 687 WP_003155022.1 lytic transglycosylase domain-containing protein -
  HWH77_RS16350 (HWH77_16355) - 3215029..3215604 (-) 576 WP_015388447.1 CYTH domain-containing protein -
  HWH77_RS16355 (HWH77_16360) - 3215729..3216094 (+) 366 WP_161503322.1 hypothetical protein -
  HWH77_RS16360 (HWH77_16365) yjbM 3216122..3216757 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=462736 HWH77_RS16325 WP_003155028.1 3210492..3212321(+) (pepF) [Bacillus velezensis strain LF01]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=462736 HWH77_RS16325 WP_003155028.1 3210492..3212321(+) (pepF) [Bacillus velezensis strain LF01]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCTTTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCACATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCACCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATATGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTAAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCGTTATCCAA
TAACAGCATTCCTGAAGAAGTTTACGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTAATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGCCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACGG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGGGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488